Identification of the major intestinal fatty acid transport protein
Identification of the major intestinal fatty acid transport protein
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DOI：
10.1016/s1097-2765(00)80332-9
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发表时间：
1999-09-01
期刊：
影响因子：
16
通讯作者：
Lodish, HF
中科院分区：
文献类型：
--
作者：
Stahl, A;Hirsch, DJ;Lodish, HF
虽然碳水化合物等代谢物的肠道运输系统已经被很好地表征，但脂肪酸（FA）在肠细胞顶质膜上运输的分子机制仍不清楚。在这里，我们发现FATP4是FA转运蛋白（FATPs）大家族的成员，在小肠成熟肠细胞的顶端侧高水平表达。此外，293细胞中FATP4的过表达促进了长链FAs的摄取，其特异性与肠细胞相同，而通过反义寡核苷酸降低原代肠细胞中FATP4的表达可抑制50%的FA摄取。这表明FATP4是肠细胞中主要的脂肪酸转运体，可能构成抗肥胖治疗的新靶点。
While intestinal transport systems for metabolites such as carbohydrates have been well characterized, the molecular mechanisms of fatty acid (FA) transport across the apical plasmalemma of enterocytes have remained largely unclear. Here, we show that FATP4 a member of a large family of FA transport proteins (FATPs), is expressed at high levels on the apical side of mature enterocytes in the small intestine. Further, overexpression of FATP4 in 293 cells facilitates uptake of long chain FAs with the same specificity as enterocytes, while reduction of FATP4 expression in primary enterocytes by antisense oligonucleotides inhibits FA uptake by 50%. This suggests that FATP4 is the principal fatty acid transporter in enterocytes and may constitute a novel target for antiobesity therapy.