Captive environment influences the composition and diversity of fecal microbiota in Indo‐Pacific bottlenose dolphins, Tursiops aduncus
Captive environment influences the composition and diversity of fecal microbiota in Indo‐Pacific bottlenose dolphins, Tursiops aduncus
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DOI：
10.1111/mms.12736
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发表时间：
2020-08
影响因子：
2.3
通讯作者：
A. Suzuki;Koyuki Akuzawa;Kazunobu Kogi;K. Ueda;Miwa Suzuki
中科院分区：
文献类型：
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作者：
A. Suzuki;Koyuki Akuzawa;Kazunobu Kogi;K. Ueda;Miwa Suzuki
圈养环境影响圈养动物的微生物区系；然而，野生和圈养海豚之间的微生物区系比较研究很少。为了探索圈养环境的影响，我们使用下一代测序技术对9只野生和4只圈养印度洋-太平洋宽吻海豚的粪便微生物区系进行了表征，并揭示了分析组之间粪便微生物区系的差异。在野生海豚和圈养海豚之间发现了海豚门和变形杆菌丰度的统计差异。共有36个属(占所有海豚总属的22.9%)，而野生海豚和圈养海豚共有79个属(50.3%)和42个属(26.8%)。在人工饲养的海豚体内检出包括摩根氏杆菌和支原体在内的几个致病细菌属，在野生海豚体内检出乳杆菌属。Lefse和SIMPER分析表明，圈养海豚中严格意义上的梭状芽孢杆菌属比野生海豚丰富得多，对两组之间粪便微生物区系差异的贡献最大。我们的结果表明，圈养环境影响了海豚的粪便微生物区系，并加强了监测圈养中潜在致病细菌的重要性。
Captive environments impact the microbiota of captive animals; however, the comparison of microbiota between wild and captive dolphins has been poorly investigated. To explore the impact of a captive environment, we characterized the fecal microbiota of nine wild and four captive Indo‐Pacific bottlenose dolphins,Tursiops aduncus, using a next‐generation sequencing and revealed differences in the fecal microbiota between the analyzed groups. Statistical differences in abundances of the phyla Firmicutes and Proteobacteria were found between the wild and captive dolphins. Thirty‐six genera (22.9% of the total genera detected in all dolphins) were shared between the groups, whereas 79 (50.3%) and 42 (26.8%) genera were found only in the wild or captive dolphins, respectively. Several pathogenic bacterial genera, includingMorganellaandMycoplasma, were detected only in the captive dolphins, and the genusLactobacilluswas found only in the wild dolphins. LefSe and SIMPER analyses revealed that the genusClostridiumsensu stricto 1 was significantly more abundant in the captive dolphins than in the wild ones and contributed the most to the dissimilarity of fecal microbiota between the groups. Our results indicate that the captive environment impacts the fecal microbiota of dolphins and reinforces the importance of monitoring potentially pathogenic bacteria in captivity.