Central roles of iron in the regulation of oxidative stress in the yeast Saccharomyces cerevisiae
Central roles of iron in the regulation of oxidative stress in the yeast Saccharomyces cerevisiae
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DOI：
10.1007/s00294-017-0689-4
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发表时间：
2017-03
期刊：
影响因子：
2.5
通讯作者：
Ryo Matsuo;Shogo Mizobuchi;Maya Nakashima;Kensuke Miki;D. Ayusawa;M. Fujii
中科院分区：
文献类型：
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作者：
Ryo Matsuo;Shogo Mizobuchi;Maya Nakashima;Kensuke Miki;D. Ayusawa;M. Fujii
氧对需氧生物是必需的，但可能通过产生活性氧（ROS）引起细胞毒性。在本研究中，我们筛选了酵母（saccharomyces cerevisiae）中调节氧化应激的基因，发现cth2 / tis11的表达导致对ROS的抗性增加。cth2在缺铁时上调，并具有重塑代谢以适应缺铁的功能。我们在这里发现，cth2对ROS的抗性增加可能是由于线粒体呼吸活性降低导致ROS产生减少，这与cth2下调线粒体呼吸蛋白的事实是一致的。我们还发现cth1 （cth2的类似物）的表达也引起对ROS的抵抗力增加。这一发现支持了上述观点，因为线粒体呼吸蛋白是cth1和cth2的共同靶点。我们进一步发现，培养基中添加铁可以促进s的生长。氧化应激下，cth2的表达和铁的补充共同促进了其生长。由于ecth2受铁的调控，这些发现表明铁在氧化应激inS的调控中起着至关重要的作用。酵母。
Oxygen is essential for aerobic organisms but causes cytotoxicity probably through the generation of reactive oxygen species (ROS). In this study, we screened for the genes that regulate oxidative stress in the yeastSaccharomyces cerevisiae, and found that expression ofCTH2/TIS11caused an increased resistance to ROS.CTH2is up-regulated upon iron starvation and functions to remodel metabolism to adapt to iron starvation. We showed here that increased resistance to ROS byCTH2would likely be caused by the decreased ROS production due to the decreased activity of mitochondrial respiration, which observation is consistent with the fact thatCTH2down-regulates the mitochondrial respiratory proteins. We also found that expression ofCTH1, a paralog ofCTH2, also caused an increased resistance to ROS. This finding supported the above view, because mitochondrial respiratory proteins are the common targets ofCTH1andCTH2. We further showed that supplementation of iron in medium augmented the growth ofS. cerevisiaeunder oxidative stress, and expression ofCTH2and supplementation of iron collectively enhanced its growth under oxidative stress. SinceCTH2is regulated by iron, these findings suggested that iron played crucial roles in the regulation of oxidative stress inS. cerevisiae.