TOPOGRAPHICAL ORGANIZATION OF EFFERENT PROJECTIONS FROM THE CAT SUBSTANTIA NIGRA PARS RETICULATA
TOPOGRAPHICAL ORGANIZATION OF EFFERENT PROJECTIONS FROM THE CAT SUBSTANTIA NIGRA PARS RETICULATA
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DOI：
10.1016/0006-8993(88)90090-x
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发表时间：
1988-07-12
期刊：
影响因子：
2.9
通讯作者：
BESSON, MJ
中科院分区：
文献类型：
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作者：
KEMEL, ML;DESBAN, M;BESSON, MJ
本文用辣根过氧化物酶-小麦凝集素（HRP-WGA）和标记蛋白质的顺行运输，研究了猫黑质（SN）向丘脑和上级丘传出投射的地形结构。将HRP-WGA或[14 C]氨基酸的混合物注射到SN的各个区域中，并分别通过组织化学或放射自显影术在冠状脑切片上可视化运输的材料。[14 C] γ的逆行运输氨基丁酸（[14 C]GABA）注射到丘脑核也被用来确定黑质丘脑投射的身份。使用HRP-WGA或标记蛋白质的顺行运输发现相同的结果。在丘脑中，密集的黑质投射观察到腹内侧核（VM）和腹外侧核（VL）的吻中腹侧部分，而更有限的投射被认为是在中央外侧核（CL）和中央旁核（PC）以及在内侧背核（MD-II）的间室区。此外，在上级丘的中间层中发现了HRP-WGA或放射性的斑片状分布。更确切地说，标记的VM是密集的[14 C]氨基酸注射到中间部分的SN部网状（SNR），无论注射部位的深度，而intralaminar核标记优先注射到背侧部分的中间SNR。当[14 C]氨基酸被注射到SNR的吻侧部分时，在整个尾侧和侧吻侧范围内观察到黑质投射到上级丘的中间层。标记的上级丘也被视为注射后的[14 C]氨基酸的SNR的中间部分，但在这种情况下，腹侧注射导致更强烈的标签比背侧的。当[14 C]GABA注入丘脑VM核时，SNR和SN丘脑部（SNC）均被标记，证实了黑质VM投射是GABA能的，并表明这些GABA能细胞的神经支配SNC的循环侧枝也含有转运的放射性物质。在这种情况下（[14 C]GABA注射到VM），丘脑网状核也表现出密集的标记。另一方面，当[14 C]GABA注入CL或MD-II时，在SN（SNR或SNC）中未观察到标记，表明黑质投射到这些丘脑核团是非GABA能的。然而，[14 C]GABA注入CL标记丘脑网状核和上级丘，表明这些结构发送GABA能投射到CL。
The topographical organization of the efferent projections from the cat substantia nigra (SN) to the thalamus and the superior colliculus was examined using the anterograde transport of horseradish peroxidase-wheat agglutinin (HRP-WGA) and of labelled proteins. HRP-WGA or a mixture of [14C]amino acids was injected into various areas of the SN and the transported material visualized on coronal brain sections by histochemistry or autoradiography, respectively. The retrograde transport of [14C].gamma.-aminobutyric acid ([14C]GABA) injected into thalamic nuclei was used also to determine the identity of the nigrothalamic projections. Identical results were found using either the anterograde transport of HRP-WGA or of labelled proteins. In the thalamus, dense nigral projections were observed in the nucleus ventralis medialis (VM) and in the rostromedioventral part of the nucleus ventralis lateralis (VL) whilst more limited projections were seen in the nuclei centralis lateralis (CL) and paracentralis (PC) as well as in the paralamellar zone of the nucleus medialis dorsalis (MD-II). In addition, a patchy, distribution of HRP-WGA or of radioactivity was found in the intermediate layer of the superior colliculus. More precisely, labelling of the VM was dense following injection of [14C] amino acids into the intermediate part of the SN pars reticulata (SNR) regardless of the depth of the injection site, whilst the intralaminar nuclei were labelled preferentially following injections made into the dorsal part of the intermediate SNR. Nigral projections to the intermediate layer of the superior colliculus were visualized over the whole mediocaudal and laterorostral extent when [14C] amino acids were injected into the rostral part of the SNR. Labelling of the superior colliculus was also seen following injection of [14C] amino acids into the intermediate part of the SNR but, in this case, ventral injections led to a more intense labelling than dorsal ones. Both the SNR and the SN pars compacta (SNC) were labelled when [14C]GABA was injected into the VM nucleus of the thalamus, confirming that the nigro VM projection is GABAergic and showing that recurrent collaterals of these GABAergic cells innervating the SNC also contained the transported radioactive material. In this condition ([14C]GABA injection into the VM), the thalamic reticularis nucleus also exhibited a dense labelling. On the other hand, no labelling was observed in the SN (either SNR or SNC) when [14C]GABA was injected into the CL or the MD-II, suggesting that the nigral projections to these thalamic nuclei are non-GABAergic. However, [14C]GABA injection into the CL did label the thalamic reticularis nucleus and the superior colliculus, suggesting that these structures send GABAergic projections to the CL.