Mice lacking leukocyte common antigen-related (LAR) protein tyrosine phosphatase domains demonstrate spatial learning impairment in the two-trial water maze and hyperactivity in multiple behavioural tests
Mice lacking leukocyte common antigen-related (LAR) protein tyrosine phosphatase domains demonstrate spatial learning impairment in the two-trial water maze and hyperactivity in multiple behavioural tests
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DOI：
10.1016/j.bbr.2004.02.006
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发表时间：
2004-09-23
影响因子：
2.7
通讯作者：
Van der Zee, CEEM
中科院分区：
文献类型：
--
作者：
Kolkman, MJM;Streijger, F;Van der Zee, CEEM
白细胞共同抗原相关蛋白(LAR)是一种细胞黏附分子样受体样蛋白酪氨酸磷酸酶。我们先前报道，在LAR酪氨酸磷酸酶缺陷(LAR-DeltaP)小鼠中，基底前脑胆碱能神经元的数量和大小及其在海马区的神经支配减少。由于海马体涉及行为活动方面，包括学习和记忆过程，我们使用一系列啮齿动物行为测试来评估LAR磷酸酶缺乏的可能表型后果。运动功能和协调能力测试以及空间学习能力测试没有显示野生型和LAR-DeltaP小鼠之间的任何表现差异。在Morris水迷宫的困难变体中发现了空间学习障碍。探索、筑巢和活动测试表明，LAR-DeltaP小鼠比野生型小鼠更活跃。观察到的LAR-DeltaP小鼠的过度活动不能用焦虑或好奇心水平的变化来解释，而且被发现在整个夜间持续存在。总而言之，对LAR-DeltaP小鼠的行为测试显示出空间学习障碍和活动显著增加。(C)2004爱思唯尔B.V.保留所有权利。
Leukocyte common antigen-related (LAR) protein is a cell adhesion molecule-like receptor-type protein tyrosine phosphatase. We previously reported that in LAR tyrosine phosphatase-deficient (LAR-DeltaP) mice the number and size of basal forebrain cholinergic neurons as well as their innervation of the hippocampal area was reduced. With the hippocampus being implicated in behavioural activity aspects, including learning and memory processes, we assessed possible phenotypic consequences of LAR phosphatase deficiency using a battery of rodent behaviour tests. Motor function and co-ordination tests as well as spatial learning ability assays did not reveal any performance differences between wildtype and LAR-DeltaP mice. A spatial learning impairment was found in the difficult variant of the Morris water maze. Exploration, nestbuilding and activity tests indicated that LAR-DeltaP mice were more active than wildtype littermates. The observed hyperactivity in LAR-DeltaP mice could not be explained by altered anxiety or curiosity levels, and was found to be persistent throughout the nocturnal period. In conclusion, behavioural testing of the LAR-DeltaP mice revealed a spatial learning impairment and a significant increase in activity. (C) 2004 Elsevier B.V. All rights reserved.