Theoretical model for the formation of caveolae and similar membrane Invaginations
Theoretical model for the formation of caveolae and similar membrane Invaginations
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DOI：
10.1016/s0006-3495(04)74266-6
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发表时间：
2004-04-01
影响因子：
3.4
通讯作者：
Turner, MS
中科院分区：
文献类型：
--
作者：
Sens, P;Turner, MS
我们研究了流体脂膜在张力作用下形成芽状内陷的物理模型，并将该模型应用于小窝的形成。我们证明，出芽可以由膜结合的蛋白质驱动，前提是它们在膜上施加不对称力，从而产生弯矩。特别是，小窝的形成不一定需要细胞骨架施加力量。我们的理论模型能够解释在小窝中实验观察到的几个特征，已知小窝家族中的蛋白质在小窝芽的形成中发挥关键作用。这包括1)形成大小在100纳米范围内的小凹芽和2)某些N-末端和C-末端缺失突变导致小泡大一个数量级。最后，我们讨论了在小窝表面观察到的形态条纹的可能来源。
We study a physical model for the formation of bud-like invaginations on fluid lipid membranes under tension, and apply this model to caveolae formation. We demonstrate that budding can be driven by membrane-bound proteins, provided that they exert asymmetric forces on the membrane that give rise to bending moments. In particular, caveolae formation does not necessarily require forces to be applied by the cytoskeleton. Our theoretical model is able to explain several features observed experimentally in caveolae, where proteins in the caveolin family are known to play a crucial role in the formation of caveolae buds. These include 1), the formation of caveolae buds with sizes in the 100-nm range and 2), that certain N- and C-termini deletion mutants result in vesicles that are an order-of-magnitude larger. Finally, we discuss the possible origin of the morphological striations that are observed on the surfaces of the caveolae.