DNA-SEQUENCES OF YEAST-H3 AND YEAST-H4 HISTONE GENES FROM 2 NON-ALLELIC GENE SETS ENCODE IDENTICAL H3-PROTEINS AND H4-PROTEINS
DNA-SEQUENCES OF YEAST-H3 AND YEAST-H4 HISTONE GENES FROM 2 NON-ALLELIC GENE SETS ENCODE IDENTICAL H3-PROTEINS AND H4-PROTEINS
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DOI：
10.1016/s0022-2836(83)80164-8
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发表时间：
1983-01-01
影响因子：
5.6
通讯作者：
ANDRESSON, OS
中科院分区：
文献类型：
--
作者：
SMITH, MM;ANDRESSON, OS
测定了酿酒葡萄球菌H3和H4组蛋白2个位点的完整DNA序列。每个位点包含1个H3和1个H4基因。每个位点上的基因是发散转录的，编码序列在一个位点上有646个碱基对，在另一个位点上有676个碱基对。H3基因编码相同的组蛋白H3蛋白，H4基因编码相同的组蛋白H4蛋白。酵母蛋白与小牛的组蛋白H3和H4分别有15个和8个氨基酸的取代，这些差异主要局限于蛋白质的羧基末端。这些基因在同义密码子使用方面表现出与其他酵母基因相似的偏见。这种偏差仅限于基因的编码序列，并且对编码蛋白质的阅读框具有特异性。每个基因的编码序列两侧都有A + T含量为70-80%的DNA。可能的调控序列位于组蛋白H3和H4 RNA转录本的5‘和3’端。
The complete DNA sequences of 2 loci encoding H3 and H4 histones in S. cerevisiae were determined. Each locus contains 1 H3 and 1 H4 gene. The genes at each locus are divergently transcribed and the coding sequences are separated by 646 base-pairs at one locus and 676 base-pairs at the other. The H3 genes code for identical histone H3 proteins and the H4 genes code for identical histone H4 proteins. The yeast proteins differ from histones H3 and H4 of calf by 15 and 8 amino acid substitutions, respectively, and these differences are largely confined to the carboxy-terminal halves of the proteins. The genes demonstrate a bias in synonymous codon usage similar to that noted for other yeast genes. This bias is confined to the coding sequences of the genes and is specific for the reading frame encoding the proteins. The coding sequence of each gene is flanked on both sides by DNA with an A + T content of 70-80%. Possible regulatory sequences are located relative to the 5'' and 3''-termini of the histone H3 and H4 RNA transcripts.