Production of borreliacidal antibody to outer surface protein A in vitro and modulation by interleukin-4
Production of borreliacidal antibody to outer surface protein A in vitro and modulation by interleukin-4
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DOI：
10.1128/iai.68.10.5496-5501.2000
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发表时间：
2000-10-01
影响因子：
3.1
通讯作者：
Schell, RF
中科院分区：
文献类型：
--
作者：
Munson, EL;Du Chateau, BK;Schell, RF
杀疏螺旋体抗体产生是确定伯氏疏螺旋体疫苗有效性的几个参数之一。用巨噬细胞和B螺旋体体外培养免疫淋巴结细胞，研究其产生抗疏螺旋体抗体的能力。我们表明，主要针对外表面蛋白A（OspA）的杀疏螺旋体抗体很容易由从接种福尔马林灭活的B、氢氧化铝中的伯氏螺旋体的C3 H/HeJ小鼠获得的淋巴结细胞产生，但不是重组OspA，在接种后第7天获得的淋巴结细胞培养物上清液中检测到抗OspA杀疏螺旋体抗体，在第17天达到峰值，然后迅速下降。杀疏螺旋体活性归因于免疫球蛋白G1（IgG 1）、IgG 2a和IgG 2b抗体。当淋巴结细胞用白细胞介素-4（IL-4）处理时，杀疏螺旋体抗体的产生受到抑制，但不受抗IL-4抗体处理的影响。这些结果表明，其他细胞因子，而不是IL-4，主要负责产生的二次杀疏螺旋体抗体反应。
Borreliacidal antibody production is one of several parameters for establishing the effectiveness of Borrelia burgdorferi vaccines. The production of borreliacidal antibody was studied in vitro by culturing immune lymph node cells with macrophages and B, burgdorferi. We showed that borreliacidal antibody, directed primarily against outer surface protein A (OspA), was readily produced by lymph node cells obtained from C3H/HeJ mice vaccinated with formalin-inactivated B, burgdorferi in aluminum hydroxide, but not recombinant OspA, Anti-OspA borreliacidal antibody was detected in supernatants of cultures of lymph node cells obtained on day 7 after vaccination, peaked on day 17, and rapidly declined. The borreliacidal activity was attributable to immunoglobulin G1 (IgG1), IgG2a, and IgG2b antibodies. When lymph node cells were treated with interleukin-4 (IL-4), production of borreliacidal antibody was inhibited but was unaffected by treatment with anti-IL-4 antibodies. These results suggest that other cytokines, but not IL-4, are mainly responsible for production of the secondary borreliacidal antibody response.