Non-destructive staging of barley reproductive development for molecular analysis based upon external morphology.
Non-destructive staging of barley reproductive development for molecular analysis based upon external morphology.
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DOI：
10.1093/jxb/ers092
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发表时间：
2012-06
影响因子：
6.9
通讯作者：
Wilson ZA
中科院分区：
文献类型：
--
作者：
Gómez JF;Wilson ZA
研究花粉和花药发育的分子遗传途径的先决条件是精确的生殖发育分期系统。然而，在大麦中，小花形成主要发生在假茎内，这使得在不解剖植物的情况下观察小花发育和接近小花特别困难。因此，选择用于分子分析的阶段不能非破坏性地进行。因此，已经开发了大麦的分期方法，以确定容易检测的生长点和生殖发育之间的关系，提供一个明确的关键，使生殖材料的准确选择。最初的分期遵循传统的扎多克十进制系统，对第31-34期和第37期进行了微小的调整。后期阶段，从37起，被取代的生长分期的基础上最后的旗伸长（LFE）和假茎内的穗所占据的位置。通过使用包含Zadoks分期31-37的分期系统，辅以子分期和LFE分期以提高准确性，可以很容易地预测尖峰大小。不同的穗大小，以及LFE阶段，表现出明确的关系，花药内发生的事件，证实了由光学显微镜的花药。穗大小和发育到花药发育之间的确定关系现在使得通过外部分期准确预测花药和花粉发育成为可能。因此，这提供了一种非破坏性选择材料进行分析的机制，这对于花药和花粉发育中基因的分子表征至关重要。
A prerequisite to study the molecular genetic pathways of pollen and anther development is an accurate staging system for reproductive development. However in barley, floret formation occurs mainly within the pseudostem, which makes the observation of floret development and access to the floret particularly difficult without dissecting the plant. Thus selecting stages for molecular analysis cannot be done non-destructively. A staging method has therefore been developed for barley in order to define the relationship between readily detectable growth points and reproductive development, to provide a clear key to enable accurate selection of reproductive material. Initial staging followed the traditional Zadoks decimal system, with minor adaptations to stages 31–34 and stage 37. The later stages, from 37 onward, were replaced by growth staging based upon the last flag elongation (LFE) and the position occupied by the spike within the pseudostem. Spike size could be readily predicted by using the staging system incorporating Zadoks stages 31–37, supplemented with substages and by LFE staging to improve accuracy. The different spike sizes, as well as the LFE stages, showed a clear relationship to events occurring within the anther, as confirmed by light microscopy of the anthers. The defined relationship between spike size and development to anther development now makes possible the accurate prediction of anther and pollen progression by external staging. This, therefore, provides a mechanism for non-destructive selection of material for analysis that is critical for the molecular characterization of genes in anther and pollen development.
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影响因子：
2
作者：
SAVIN, R;SLAFER, GA
通讯作者：
SLAFER, GA
影响因子：
6.9
作者：
Reynolds, Matthew;Foulkes, M. John;Angus, William J.
通讯作者：
Angus, William J.
影响因子：
5.8
作者：
Demotes-Mainard, S;Jeuffroy, MH
通讯作者：
Jeuffroy, MH
影响因子：
2
作者：
Arduini, Iduna;Ercoli, Laura;Masoni, Alessandro
通讯作者：
Masoni, Alessandro
影响因子：
7.2
作者：
Caldwell, DG;McCallum, N;Waugh, R
通讯作者：
Waugh, R