PRODUCTION OF TRANSGENIC RABBITS, SHEEP AND PIGS BY MICROINJECTION
PRODUCTION OF TRANSGENIC RABBITS, SHEEP AND PIGS BY MICROINJECTION
复制标题
DOI：
10.1038/315680a0
复制
发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
BRINSTER, RL
中科院分区：
文献类型：
--
作者：
HAMMER, RE;PURSEL, VG;BRINSTER, RL
直接显微注射已被用于将外源DNA导入许多终末分化的细胞类型以及几个物种的胚胎中，包括海胆1、秀丽假丝酵母2、Xenopus 3、果蝇4、5和小鼠6 -11。已经成功地将各种基因引入小鼠，包括由与大鼠或人生长激素（hGH）结构基因融合的小鼠金属硫蛋白-I（MT）启动子/调节子区域组成的构建体。携带这种基因的转基因小鼠通常在几个器官中表现出高的金属诱导水平的融合信使RNA，血清中大量的外源生长激素和增强的生长12，13。此外，该基因稳定地整合到生殖系中，使表型可遗传。鉴于转基因家畜的重要科学意义和潜在的经济价值，我们在大型动物上开展了研究，将融合基因MT-hGH 13显微注射到超数排卵的兔、羊和猪的卵原核或卵核中。我们在这里报告的基因整合在所有三个物种和表达的基因在转基因兔和猪。
Direct microinjection has been used to introduce foreign DNA into a number of terminally differentiated cell types as well as embryos of several species including sea urchin1,Candida elegans2,Xenopus3,Drosophila4,5and mice6–11. Various genes have been successfully introduced into mice including constructs consisting of the mouse metallothionein-I (MT) promoter/regulator region fused to either the rat or human growth hormone (hGH) structural genes. Transgenic mice harbouring such genes commonly exhibit high, metal-inducible levels of the fusion messenger RNA in several organs, substantial quantities of the foreign growth hormone in serum and enhanced growth12,13. In addition, the gene is stably incorporated into the germ line, making the phenotype heritable. Because of the scientific importance and potential economic value of transgenic livestock containing foreign genes, we initiated studies on large animals by microinjecting the fusion gene,MT–hGH13, into the pronuclei or nuclei of eggs from superovulated rabbits, sheep and pigs. We report here integration of the gene in all three species and expression of the gene in transgenic rabbits and pigs.