RAPID, SIMULTANEOUS DETERMINATION OF ISOMERS OF RETINAL, RETINAL OXIME AND RETINOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
RAPID, SIMULTANEOUS DETERMINATION OF ISOMERS OF RETINAL, RETINAL OXIME AND RETINOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0021-9673(00)90269-3
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发表时间：
1988-04-22
期刊：
影响因子：
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通讯作者：
OLSON, JA
中科院分区：
文献类型：
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作者：
LANDERS, GM;OLSON, JA
视黄醇、视黄醇和视黄酮肟的13个几何异构体在两个250倍的单层析中被拆分。4 mm 5-m LiChrosorb Si60色谱柱，流动相为11.2%乙酸乙酯、1.4%正辛醇和2%正己烷。视黄醇和视黄醇的11-顺式和全反式异构体以及9-顺式和13-顺式异构体均被完全拆分。这种层析方法对于定量生物样品(如眼组织)的二氯甲烷-羟胺提取物中的维甲酸几何异构体特别有用。
Thirteen geometric isomers of retinol, retinal and retinal oxime were resolved in a single chromatographic run on two 250 .times. 4 mm 5-.mu.m LiChrosorb Si 60 columns in series, using 11.2% ethyl acetate, 1.4% 1-octanol and 2% dioxane in hexane as the mobile phase. All the 11-cis and all-trans isomers of retinol and retinal oxime were completely resolved from each other and from the 9-cis and 13-cis isomers. This chromatographic procedure should be particularly useful for quantifying geometric isomers of retinoids in methylene chloride-hydroxylamine extracts of biological samples such as ocular tissues.