Inflammation related to high-mobility group box-1 in endometrial ovarian cyst
Inflammation related to high-mobility group box-1 in endometrial ovarian cyst
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DOI：
10.1016/j.jri.2021.103292
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发表时间：
2021-02-26
影响因子：
3.4
通讯作者：
Takeshita, Toshiyuki
中科院分区：
文献类型：
--
作者：
Ikeda, Mariko;Negishi, Yasuyuki;Takeshita, Toshiyuki
子宫内膜异位症是一种慢性炎症性疾病，常伴有痛经、不孕症、子宫腺肌症和子宫内膜卵巢囊肿(EOC)。特别是，在纵向随访中，EoC有时会变得恶性。本研究旨在探讨高迁移率族蛋白-1(HMGB1)在上皮性卵巢癌炎症环境中的作用及免疫细胞的特点。标本取自卵巢良性囊肿术后患者。将受试者分为两组：EoC组(EoC组)和非EoC组(nEoC组)。我们将摘除的卵巢的一部分分割成小段，并分离出组织细胞。然后，用流式细胞仪分析DC、巨噬细胞和非(I)免疫细胞的胞浆HMGB1水平。我们还评估了免疫、T、NK、iNKT、NK和调节性T(Treg)细胞的比例。结果表明，EOC的DC、巨噬细胞和非免疫细胞胞浆HMGB1水平显著高于NEOC。CD69和CD107a在CD8(+)T细胞和CD4(+)T细胞上的表达也明显增强。此外，M2巨噬细胞和Tregs在EoC中高度聚集。这些结果表明，HMGB1可加重与T细胞活化有关的慢性炎症，同时促进EOCs免疫抑制环境的形成。
Endometriosis is a chronic inflammatory disease often associated with dysmenorrhea, infertility, adenomyosis, and endometrial ovarian cyst (EOC). In particular, EOC can sometimes become malignant in a longitudinal follow-up. This study aimed to investigate the involvement of high-mobility group box-1 (HMGB1) in an inflammatory milieu and the characteristics of immune cells in EOC. The samples were obtained from patients who underwent ovarian cystectomy for benign ovarian cyst. The participants were divided into two groups: patients with EOC (EOC group) and those without EOC (nEOC group). We divided a part of the removed ovary into small sections and isolated the tissue cells. Thereafter, the cytoplasmic HMGB1 levels in DCs, macrophages, and non-(i)mmune cells were analyzed by flow cytometry. We also evaluated the proportions of immune, T, NK, iNKT, NK, and regulatory T (Treg) cells. Results showed that the DCs, macrophages, and non-immune cells of EOC had significantly higher cytoplasmic HMGB1 levels than those of nEOC. The expression of CD69 and CD107a on CD8(+) T and CD4(+) T cells of EOC was also more enhanced than that of nEOC. Furthermore, the M2 macrophages and Tregs highly accumulated in EOC. These results indicate that HMGB1 may aggravate chronic inflammation related to T-cell activation and simultaneously facilitate development of the immunosuppressive milieu in EOCs.