Developmental changes in tritium autoabsorption.
Developmental changes in tritium autoabsorption.
复制标题
氚自吸收的发育变化。
DOI：
10.1016/1053-8119(92)90003-6
复制
发表时间：
1992
期刊：
影响因子：
5.7
通讯作者：
Lothman,EW
中科院分区：
文献类型：
--
作者：
Harrison,MB;Hogan,CJ;Lothman,EW
Developmental changes in autoabsorption of tritium emissions were examined in 30 brain regions in the rat at Postnatal Days 0, 4, 7, 10, 14, 21, and 28 and adulthood. Rats received tritiated 2-deoxyglucosein vivo. Alternate brain sections were extracted in chloroform, and autoradiographs were developed from extracted and nonextracted sections. The ratio of optical density values in extracted vs nonextracted sections was used to determine autoabsorption for each structure. Three principal temporal patterns in the development of adult levels of autoabsorption, determined by the optical density ratios, were identified: (1)a minimal increase patternin which autoabsorption rose only slightly between birth and adulthood; (2)a plateau patternin which a rapid early increase was followed by stable values; and (3)a late increase patternin which autoabsorption remained relatively constant until Postnatal Day 28, with a large increase between Day 28 and adulthood. In addition, optical density ratios fluctuated during the second postnatal week in close to one-third of the structures. The data suggest that developmental events affecting the ratio of gray to white matter produce substantial local variations in the development of adult levels of autoabsorption that are distinct for each structure. To correct for autoabsorption effects in ontogenetic studies using tritium autoradiography, it is necessary to determine directly the degree of autoabsorption at a particular time point for the structure of interest. Our results indicate that the technique ofin vivoadministration of tritiated 2-deoxyglucose followed by chloroform extraction appears to be a sensitive and reproducible method for assessing autoabsorption at all ages.
