Identification of annexin A1 as a proinvasive and prognostic factor for lung adenocarcinoma
Identification of annexin A1 as a proinvasive and prognostic factor for lung adenocarcinoma
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鉴定膜联蛋白 A1 作为肺腺癌的促侵袭和预后因素
DOI：
10.1007/s10585-011-9380-1
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发表时间：
2011-06-01
影响因子：
4
通讯作者：
Chen, Zhu-Chu
中科院分区：
文献类型：
--
作者：
Liu, Ying-Fu;Zhang, Peng-Fei;Chen, Zhu-Chu
转移是肺癌患者最常见的死亡原因，也是成功治疗的主要障碍。为了发现肺腺癌 (AdC) 中新的转移相关蛋白，对有淋巴结转移 (LNM AdC) 和无淋巴结转移 (non-LNM AdC) 的原发性肺 AdC 组织进行定量蛋白质组分析。在这项研究中，与非 LNM AdC 相比，膜联蛋白 A1 在 LNM AdC 中显着上调。免疫组织化学显示，与非 LNM AdC 相比，在 LNM AdC 和匹配的淋巴结转移中经常观察到膜联蛋白 A1 过度表达。膜联蛋白 A1 过表达与肺 AdC 中的晚期临床分期 (P<0.05) 和淋巴结转移 (P<0.05) 以及复发率增加 (P<0.05) 和总生存率降低 (P<0.05) 显着相关。 Cox回归分析表明膜联蛋白A1过度表达是一个独立的预后因素。此外，通过siRNA干扰抑制annexin A1表达可显着抑制体外肺腺癌细胞A549的侵袭能力。综上所述，annexin A1表达与肿瘤分期、淋巴结转移、复发和患者生存相关。膜联蛋白 A1 被认为在肺 AdC 的进展中发挥重要作用。
Metastasis is the most common cause of death in lung cancer patients and is a major obstacle to the successful treatment. To discover novel metastasis-related proteins in lung adenorcinoma (AdC), quantitative proteomic analysis was performed between primary lung AdC tissues with (LNM AdC) and without lymph node metastasis (non-LNM AdC). In this study, annexin A1 was identified to be significantly up-regulated in LNM AdC compared with non-LNM AdC. Immunohistochemistry showed that annexin A1 over-expression was frequently observed in LNM AdCs and matched lymph node metastases compared with non-LNM AdCs. Annexin A1 over-expression was significantly associated with advanced clinical stage (P< 0.05) and lymph node metastasis (P< 0.05) and increased relapse rate (P< 0.05) and decreased overall survival (P< 0.05) in lung AdCs. Cox regression analysis indicated annexin A1 over-expression was an independent prognostic factor. Furthermore, suppression of annexin A1 expression by siRNA interference significantly inhibited the invasion ability of lung adenocarcinoma cell A549 in vitro.In conclusion, annexin A1 expression correlated with tumor stage, lymph node metastasis, relapse, and patient survival. Annexin A1 is proposed to function importantly in the progression of lung AdC.