Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation.
Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation.
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DOI：
10.1364/boe.440444
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发表时间：
2021-11-01
影响因子：
3.4
通讯作者：
Yasuno Y
中科院分区：
文献类型：
--
作者：
El-Sadek IA;Miyazawa A;Shen LT;Makita S;Mukherjee P;Lichtenegger A;Matsusaka S;Yasuno Y
我们提出了一种完全无标记的三维（3D）光学相干断层扫描（OCT）为基础的组织动力学成像方法的可视化和量化的代谢和坏死活动的肿瘤球体。我们的方法是基于自定义的3D扫描协议，该协议旨在仅在几十秒内捕获体积组织动力学断层扫描图像。将该方法应用于肿瘤球体的评价。对球体的时间过程活力变化和抗癌药物反应进行定性可视化和定量分析。OCT的动态图像和荧光显微镜图像之间的相似性也得到了证明。
We present a completely label-free three-dimensional (3D) optical coherence tomography (OCT)-based tissue dynamics imaging method for visualization and quantification of the metabolic and necrotic activities of tumor spheroid. Our method is based on a custom 3D scanning protocol that is designed to capture volumetric tissue dynamics tomography images only in a few tens of seconds. The method was applied to the evaluation of a tumor spheroid. The time-course viability alteration and anti-cancer drug response of the spheroid were visualized qualitatively and analyzed quantitatively. The similarity between the OCT-based dynamics images and fluorescence microscope images was also demonstrated.