Peroxisome proliferator-activated receptor alpha is involved in the temperature-induced sex differentiation of a vertebrate
Peroxisome proliferator-activated receptor alpha is involved in the temperature-induced sex differentiation of a vertebrate
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DOI：
10.1038/s41598-020-68594-y
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发表时间：
2020-07-15
影响因子：
4.6
通讯作者：
Kitano, Takeshi
中科院分区：
文献类型：
--
作者：
Hara, Seiji;Furukawa, Fumiya;Kitano, Takeshi
青鳉（Oryzias latipes）是一种硬骨鱼，具有与哺乳动物相似的XX/XY性别决定系统。然而，在高温条件下，XX青鳉是雄性化的升高皮质醇，主要硬骨鱼糖皮质激素。在这项研究中，以确定新的因素在性腺作用的下游皮质醇在性分化过程中，我们进行了RNA测序（RNA-seq）分析，使用在正常温度下饲养的幼虫的性腺区域有和没有皮质醇，并在高温下。RNA-seq和实时PCR分析表明，皮质醇增加了一些过氧化物酶体增殖物激活受体α（PPAR α）信号靶向基因的表达。在某些情况下，过氧化物酶体增殖物激活受体α激动剂治疗诱导XX青鳉的雄性化，并与皮质醇的激动剂的共同治疗进一步诱导雄性化，而与皮质醇或激动剂的ppara敲除青鳉的治疗没有诱导雄性化。这项研究提供了第一个证据表明，过氧化物酶体增殖物激活受体α参与环境性别决定的脊椎动物。
Medaka (Oryzias latipes) is a teleost fish with an XX/XY sex determination system, similar to that of mammals. However, under high temperature conditions, XX medaka is masculinised by elevation of cortisol, the major teleost glucocorticoid. In this study, to identify novel factors in the gonads acting downstream from cortisol during sexual differentiation, we performed RNA sequencing (RNA-seq) analysis using the gonadal regions of larvae reared at normal temperature with and without cortisol, and at high temperature. The RNA-seq and real-time PCR analyses showed that expression of some peroxisome proliferator-activated receptor alpha (PPAR alpha) signalling-targeted genes was increased by cortisol. PPAR alpha agonist treatment induced masculinisation of XX medaka in some cases, and co-treatment of the agonist with cortisol further induced masculinisation, whereas treatment of pparaa knockout medaka with cortisol or the agonist did not induce masculinisation. This study provides the first evidence that PPAR alpha is involved in environmental sex determination in vertebrates.