Properties of an epithelial cell type in culture: the epidermal keratinocyte and its dependence on products of the fibroblast.
Properties of an epithelial cell type in culture: the epidermal keratinocyte and its dependence on products of the fibroblast.
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培养中上皮细胞类型的特性：表皮角质形成细胞及其对成纤维细胞产物的依赖性。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
T. Sun
中科院分区：
文献类型：
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作者：
H. Green;J. Rheinwald;T. Sun
层状鳞状上皮的角质形成细胞可以在培养中连续生长，并保留其分化形式的各种典型标记。为了在每次移植中形成集落，角质形成细胞必须得到成纤维细胞的适当支持。畸胎瘤来源的已建立的角化细胞系表现出这种依赖性，从人类皮肤中获得有限培养寿命的二倍体菌株也是如此。至少对某些角化细胞系来说，成纤维细胞形成的可溶性产物可以满足这一要求。这表明上皮细胞通常不是独立的细胞类型，其培养能力差可能是由于未能提供合适的成纤维细胞支持。存在许多已建立的上皮来源系可以在没有这种支持的情况下生长，而纤维母细胞来源系不能支持角质形成细胞，这表明在已建立的细胞系中，上皮依赖性和成纤维细胞支持功能有时会丧失。
Keratinocytes of stratified squamous epithelium can be grown serially in culture and retain the various markers typical of their form of differentiation. In order to form colonies at each transfer, the keratinocytes must be suitably supported by fibroblasts. Established keratinocyte lines of teratomal origin show this dependence, as do diploid strains of finite culture life derived from human skin. For at least some keratinocyte lines, this requirement can be satisfied by soluble products elaborated by the fibroblasts. It is suggested that epithelial cells in general may not be independent cell types and that their poor cultivability may be due to failure to provide suitable fibroblast support. The existence of a number of established lines of epithelial origin that can grow without such support and of lines of fibroblastic origin which cannot support keratinocytes suggests that both epithelial dependence and the fibroblast supporting function can sometimes be lost in established cell lines.