INVIVO EVIDENCE THAT TRANSCRIPTION AND SPLICING ARE COORDINATED BY A RECRUITING MECHANISM
INVIVO EVIDENCE THAT TRANSCRIPTION AND SPLICING ARE COORDINATED BY A RECRUITING MECHANISM
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DOI：
10.1016/0092-8674(93)90159-n
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发表时间：
1993-04-09
期刊：
影响因子：
64.5
通讯作者：
SPECTOR, DL
中科院分区：
文献类型：
--
作者：
JIMENEZGARCIA, LF;SPECTOR, DL
我们描述了前mRNA处理组件在HeLa细胞腺病毒2感染后的核组织和它们的关系，以定位病毒RNA序列。我们观察到细胞剪接因子以及RNA聚合酶II和异质核核糖核蛋白颗粒蛋白质的病毒RNA转录位点的重新分配。在用含有β-原肌球蛋白基因的一部分的质粒瞬时转染的细胞中获得了类似的结果。我们的研究结果表明，RNA转录本和转录和前mRNA剪接因子之间有非常密切的联系，这表明这些过程在细胞核中的时间和空间上都有联系。此外，这些数据表明，募集机制，调节本地化的转录和剪接因子的启动主动转录。
We describe the nuclear organization of pre-mRNA processing components in HeLa cells upon adenovirus 2 infection and their relationship to the localization of viral RNA sequences. We observe a redistribution of cellular splicing factors as well as RNA polymerase II and heterogeneous nuclear ribonucleoprotein particle proteins to sites of viral RNA transcription. Similar results were obtained in cells transiently transfected with a plasmid containing a portion of the beta-tropomyosin gene. Our findings demonstrate a very close association between RNA transcripts and transcription and pre-mRNA splicing factors, suggesting that these processes are both temporally and spatially linked in the cell nucleus. Furthermore, these data suggest a recruiting mechanism that regulates the localization of transcription and splicing factors in response to the initiation of active transcription.