The Salivary Microbiome and Oral Cancer Risk: A Pilot Study in Fanconi Anemia
The Salivary Microbiome and Oral Cancer Risk: A Pilot Study in Fanconi Anemia
复制标题
DOI：
10.1177/0022034516678169
复制
发表时间：
2017-03-01
影响因子：
7.6
通讯作者：
Teles, F. R. F.
中科院分区：
文献类型：
--
作者：
Furquim, C. P.;Soares, G. M. S.;Teles, F. R. F.
范可尼贫血（FA）是一种罕见的遗传性疾病，其特征是染色体不稳定和DNA损伤修复受损。与一般人群相比，FA患者发生口腔鳞状细胞癌（OSCC）的时间更早，频率更高，尤其是在造血干细胞移植（HSCT）后。虽然有证据表明局部微生物组和癌变的病因学作用，但没有关于FA患者口腔微生物组的信息。本研究旨在探讨61例FA患者的唾液微生物组与口腔健康状况和OSCC危险因素的关系。填写问卷并接受临床检查后，采集唾液样本，采用V3-V4高变区16S rRNA测序进行分析。使用一般线性模型分析与医学和临床参数相关的微生物概况。患者年轻（平均年龄22岁），大多数接受过HSCT （n = 53）。丰度最高的门是厚壁菌门（平均相对丰度为42.1%）和拟杆菌门（平均相对丰度为11.4%）。移植物抗宿主病（GVHD）病史（n = 27）与厚壁菌门比例较高相关（43.8% x 38.5%, P = 0.05）。高水平牙龈出血与普氏菌属（22.25% × 20%）、链球菌（19.83% × 17.61%）、卟啉单胞菌属（3.63% × 1.42%, P = 0.03）、梅毒螺旋体（1.02% × 0.28%, P = 0.009）、细小单胞菌属（0.28% × 0.07%, P = 0.02）和Dialister （0.27% × 0.10%, P = 0.04）相关。最后，11年前移植的参与者显示出最高水平的链球菌（18.4%），嗜血杆菌（12.7%）和奈瑟菌（6.8%）。总之，口腔卫生较差的FA患者具有较高比例的与牙龈疾病相容的细菌属。特定的微生物差异与口腔GVHD史和口腔黏膜炎史相关。
Fanconi anemia (FA) is a rare genetic disease characterized by chromosomal instability and impaired DNA damage repair. FA patients develop oral squamous cell carcinoma (OSCC) earlier and more frequently than the general population, especially after hematopoietic stem cell transplantation (HSCT). Although evidence of an etiological role of the local microbiome and carcinogenesis has been mounting, no information exists regarding the oral microbiome of FA patients. The aim of this study was to explore the salivary microbiome of 61 FA patients regarding their oral health status and OSCC risk factors. After answering a questionnaire and receiving clinical examination, saliva samples were collected and analyzed using 16S rRNA sequencing of the V3-V4 hypervariable region. The microbial profiles associated with medical and clinical parameters were analyzed using general linear models. Patients were young (mean age, 22 y) and most had received HSCT (n = 53). The most abundant phyla were Firmicutes [mean relative abundance (SD), 42.1% (10.1%)] and Bacteroidetes [(25.4% (11.4%)]. A history of graft-versus-host disease (GVHD) (n = 27) was associated with higher proportions of Firmicutes (43.8% x 38.5%, P = 0.05). High levels of gingival bleeding were associated with the genera Prevotella (22.25% x 20%), Streptococcus (19.83% x 17.61%), Porphyromonas (3.63% x 1.42%, P = 0.03), Treponema (1.02% x 0.28%, P = 0.009), Parvimonas (0.28% x 0.07%, P = 0.02) and Dialister (0.27% x 0.10%, P = 0.04). Finally, participants transplanted over 11 y ago showed the highest levels of Streptococcus (18.4%), Haemophilus (12.7%) and Neisseria (6.8%). In conclusion, FA patients that showed poor oral hygiene harbored higher proportions of the genera of bacteria compatible with gingival disease. Specific microbial differences were associated with a history of oral GVHD and a history of oral mucositis.