Reconstruction of small diameter arteries using decellularized vascular scaffolds.
Reconstruction of small diameter arteries using decellularized vascular scaffolds.
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DOI：
10.11480/610105
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发表时间：
2014-03
影响因子：
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通讯作者：
Yuki Nagaoka;H. Yamada;Tsuyoshi Kimura;A. Kishida;T. Fujisato;K. Takakuda
中科院分区：
文献类型：
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作者：
Yuki Nagaoka;H. Yamada;Tsuyoshi Kimura;A. Kishida;T. Fujisato;K. Takakuda
尽管有用于大直径动脉的人工血管，但尚无用于<4毫米的小直径动脉的人工血管。我们用大鼠腹动脉制作了脱细胞血管支架（长度，10 毫米；外径，1.5 毫米；内径，1.3 毫米）。我们测量了支架的生物力学特性，将其植入大鼠颈动脉的缺损处，并评估其通畅性和内皮细胞内壁。植入硅橡胶移植物作为对照。脱细胞支架表现出与正常动脉相似的机械特性。所有对照移植物均被闭塞。血栓中发现成纤维细胞样细胞，纤维组织明显。相比之下，植入后 4 周，观察到 12 只动物中有 10 只的移植物通畅。专利支架的内腔完全由内皮样细胞排列。因此，证明了使用脱细胞支架重建小动脉的可能性。
Although artificial vessels are available for large diameter arteries, there are no artificial vessels for small diameter arteries of < 4 mm. We created a decellularized vascular scaffold (length, 10 mm; outer diameter, 1.5 mm; inner diameter, 1.3 mm) from rat abdominal arteries. We measured the biomechanical characteristics of the scaffolds, implanted them to defects made in rat carotid arteries, and evaluated their patency and the endothelial cell linings. Silastic grafts were implanted as controls. The decellularized scaffolds demonstrated similar mechanical characteristics to normal arteries. All of the control grafts were occluded. Fibroblast-like cells were discovered in the thrombus, and fibrous organization was apparent. In contrast, patency of the grafts in 10 of 12 animals was observed 4 weeks after implantation. The internal cavity of the patent scaffold was completely lined by endotheliallike cells. Thus, the possibility of small artery reconstruction using decellularized scaffolds was demonstrated.