Evaluation of oxidative stress and antioxidant status: Correlation with the severity of sepsis
Evaluation of oxidative stress and antioxidant status: Correlation with the severity of sepsis
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DOI：
10.1111/sji.12653
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发表时间：
2018-04-01
影响因子：
3.7
通讯作者：
Jyoti, A.
中科院分区：
文献类型：
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作者：
Kumar, S.;Gupta, E.;Jyoti, A.
脓毒症是一种由感染引起的疾病，随后是不受调节的炎症反应，可能导致器官功能障碍。在这种情况下，氧化剂的过量产生是导致细胞毒性和最终器官衰竭和死亡的因素之一。抗氧化剂具有清除自由基的活性，对多种疾病具有保护作用。本研究旨在评估脓毒症患者的氧化和抗氧化活性水平及其与脓毒症严重程度的相关性。2016年10月至2017年6月，共有100名脓毒症患者和50名健康对照者参加了这项研究。研究包括氧化酶、髓过氧化物酶（MPO）、抗氧化酶（超氧化物歧化酶活性（SOD）和过氧化氢酶活性（CAT））和细胞因子（TNF-、IL-8和IFN-）的测定。此外，这些活动的水平与脓毒症的严重程度相关。通过DMPO硝基酮加合物的形成和血浆MPO水平活性，脓毒症患者的氧化剂水平升高（脓毒症患者为1.37 +/- 0.51，对照组为0.405 +/- 0.16）。细胞因子在败血症患者中也有所增加。血浆SOD和CAT活性显著降低(P
Sepsis is a condition caused by infection followed by unregulated inflammatory response which may lead to the organ dysfunction. During such condition, over-production of oxidants is one of the factors which contribute cellular toxicity and ultimately organ failure and mortality. Antioxidants having free radicals scavenging activity exert protective role in various diseases. This study has been designed to evaluate the levels of oxidative and antioxidative activity in sepsis patients and their correlation with the severity of the sepsis. A total of 100 sepsis patients and 50 healthy controls subjects were enrolled in this study from the period October 2016 to June 2017. The investigation included measurements of oxidative enzyme, myeloperoxidase (MPO), antioxidant enzymes including superoxide dismutase activity (SOD) and catalase activity (CAT) and cytokines (TNF-, IL-8 and IFN-). Furthermore, the level of these activities was correlated with severity of sepsis. Augmented levels of oxidants were found in sepsis as demonstrated by DMPO nitrone adduct formation and plasma MPO level activity (1.37 +/- 0.51 in sepsis vs 0.405 +/- 0.16 in control subjects). Cytokines were also found to be increased in sepsis patients. However, plasma SOD and CAT activities were significantly attenuated (P