Elucidating the Membrane Dynamics and Encapsulation Mechanism of Large DNA Molecules Under Molecular Crowding Conditions Using Giant Unilamellar Vesicles
Elucidating the Membrane Dynamics and Encapsulation Mechanism of Large DNA Molecules Under Molecular Crowding Conditions Using Giant Unilamellar Vesicles
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使用巨型单层囊泡阐明分子拥挤条件下大 DNA 分子的膜动力学和封装机制
DOI：
10.1021/acssynbio.0c00360
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发表时间：
2020
影响因子：
4.7
通讯作者：
Suzuki Hiroaki
中科院分区：
文献类型：
--
作者：
Tsugane Mamiko;Suzuki Hiroaki
在整个进化过程中，包裹基因组物质的膜结合结构的保守性表明，存在一种简单的物理机制，促进了长链DNA被脂类双层包裹。本研究旨在通过研究分子拥挤如何促进模型DNA自发包膜进入脂质双层膜来阐明这一机制。利用荧光显微镜和巨型单层囊泡(GUV)，我们发现，在GUV渗透排气和DNA-膜复合体发芽后，与模型Crowder共包裹的166kb DNA分子附着在GUV的内膜上。这组结果与耗竭体积效应是导致DNA自发包裹在GUV中的假设是一致的。这一现象可能为研究在细胞系统中发现的、独立于遗传控制的长链核酸的膜包裹的基本机制提供新的见解。
The conservation throughout evolution of membrane-bound structures that encapsulate genomic material indicates the existence of a simple, physical mechanism that facilitates the enclosing of long-stranded DNA by lipid bilayers. This study aimed to elucidate such a mechanism by investigating how molecular crowding promotes the spontaneous enveloping of model DNA into lipid bilayer membranes. Using fluorescence microscopy and giant unilamellar vesicles (GUVs) we showed that a 166 kb DNA molecule coencapsulated with a model crowder attaches to the inner membrane of the GUVs as they osmotically deflate and after the DNA–membrane complex buds out. The set of results is consistent with the hypothesis that the depletion volume effect is responsible for the spontaneous encapsulation of DNA in the GUVs. This phenomenon may offer novel insights into the basic mechanisms governing membrane encapsulation of long-stranded nucleic acids found in celluar sytems that are independent of genetic control.