Dried Blood Spheroids for Dry-State Room Temperature Stabilization of Microliter Blood Samples
Dried Blood Spheroids for Dry-State Room Temperature Stabilization of Microliter Blood Samples
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DOI：
10.1021/acs.analchem.8b01962
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发表时间：
2018-08-07
影响因子：
7.4
通讯作者：
Badu-Tawiah, Abraham K.
中科院分区：
文献类型：
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作者：
Damon, Deidre E.;Yin, Mengzhen;Badu-Tawiah, Abraham K.
众所周知，纸上的2D干血点提供了血液的简易样品收集、储存和运输。然而，大量的需求，可能的分析物不稳定性，和困难的样品回收困扰这种方法，降低分析物定量的信心。这是第一次，我们展示了一种新的方法，使用3D干燥的血液球体稳定小体积的血液样本，减轻这些影响，而无需冷藏。血液球状体在疏水纸上形成，防止样品和纸基质之间的相互作用，消除所有色谱效应。酶丙氨酸转氨酶和不稳定的有机化合物，如可卡因和安定的稳定性也被证明增加在球体中提供一个临界半径的绝缘。使用纸喷雾质谱法对干燥的血液球体进行表面分析，导致所有检测的非法药物的检测限低于ng/mL，与2D干燥血点分析相比，提高了1个数量级。
It is well-known that 2D dried blood spots on paper offer a facile sample collection, storage, and transportation of blood. However, large volume requirements, possible analyte instability, and difficult sample recovery plague this method, lowering confidence in analyte quantification. For the first time, we demonstrate a new approach using 3D dried blood spheroids for stabilization of small volume blood samples, mitigating these effects without cold storage. Blood spheroids form on hydrophobic paper, preventing interaction between the sample and paper substrate, eliminating all chromatographic effects. Stability of the enzyme alanine transaminase and labile organic compounds such as cocaine and diazepam were also shown to increase in the spheroid by providing a critical radius of insulation. On-surface analysis of the dried blood spheroids using paper spray mass spectrometry resulted in sub-ng/mL limits of detection for all illicit drugs tested, representing 1 order of magnitude improvement compared with analysis from 2D dried blood spots.