A novel IS-like element frequently inserted in a putative virulence regulator in bovine mastitis isolates of Streptococcus dysgalactiae.
A novel IS-like element frequently inserted in a putative virulence regulator in bovine mastitis isolates of Streptococcus dysgalactiae.
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一种新型的 IS 样元件经常插入停乳链球菌牛乳腺炎分离株的假定毒力调节剂中。
作者：
J. Vasi;M. Lindberg;B. Guss
停乳链球菌是一种兰斯菲尔德C群链球菌，通常从牛乳腺炎中分离。我们最近在两个S.停乳菌株8215和Epi9，由两个连续的基因dmg和dem组成，分别编码可能的调节蛋白和具有纤维蛋白原和IgG结合特性的M样蛋白。在这些研究中，发现与IS元件同源的短序列插入到菌株8215的dmg基因中。本研究描述了这个IS样元件的完整序列，命名为ISSdy1，它由1218 bp组成，包含两个开放阅读框，两侧是不完美重复序列。IS样元件的核苷酸序列与先前报道的来自变形链球菌V403的IS 199的序列显示82%的同一性。推导的ORF的氨基酸序列也揭示了高度同源性的转座酶的肠球菌屎，大肠杆菌，志贺氏菌，都属于IS 3家族的IS元件。我们研究了ISSdy 1在57个S.使用来自IS元件的特异性引物进行PCR分析，98%的分离株含有ISSdy 1元件。令人惊讶的是，在大多数研究菌株的IS样元件的副本被发现插入dmg基因，一个假定的毒力调节。
Streptococcus dysgalactiae, a Lancefield group C streptococcus, is commonly isolated from bovine mastitis. We recently identified a putative regulon in two S. dysgalactiae strains, 8215 and Epi9, consisting of two consecutive genes, dmg and dem, coding for a possible regulatory protein and an M-like protein with fibrinogen- and IgG-binding-properties, respectively. During these studies a short sequence homologous to an IS element was found to be inserted in the dmg gene of strain 8215. The present investigation describes the complete sequence of this IS-like element, named ISSdy1, which consists of 1218 bp and contains two ORFs, flanked by imperfect repeats. The nucleotide sequence of the IS-like element shows 82% identity to the previously reported sequence of IS199 from Streptococcus mutans V403. The deduced amino acid sequences of the ORFs also revealed high homology to transposases from IS elements in Enterococcus faecium, Escherichia coli, and Shigella dysenteriae, all belonging to the IS3 family. We studied the distribution of ISSdy1 in 57 S. dysgalactiae isolates using PCR analysis with specific primers derived from the IS element. Ninety-eight percent of the isolates contained the ISSdy1 element. Surprisingly, in the majority of studied strains a copy of the IS-like element was found to be inserted in the dmg gene, a putative virulence regulator.
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影响因子：
14.9
作者：
KHAN, E;MACK, JPG;SKALKA, AM
通讯作者：
SKALKA, AM
mga（化脓性链球菌中的一种调节基因）的转录分析：相位变化的单顺反子和双顺反子转录物的鉴定。
影响因子：
3.5
作者：
Bormann,NE;Cleary,PP
通讯作者：
Cleary,PP
来自变形链球菌 V403 的 IS199 的表征。
DOI：
10.1006/plas.1996.0026
发表时间：
1996
期刊：
Plasmid.
影响因子：
--
作者：
Macrina,FL;Jones,KR;Laloi,P
通讯作者：
Laloi,P
DOI：
10.1073/pnas.91.3.942
发表时间：
1994-02-01
影响因子：
11.1
作者：
DOAK, TG;DOERDER, FP;HERRICK, G
通讯作者：
HERRICK, G