Deciphering the roles of phosphoinositide lipids in phagolysosome biogenesis.
Deciphering the roles of phosphoinositide lipids in phagolysosome biogenesis.
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DOI：
10.1080/19420889.2016.1174798
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发表时间：
2016-05-01
影响因子：
--
通讯作者：
Haas, Albert
中科院分区：
文献类型：
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作者：
Jeschke, Andreas;Haas, Albert
专职吞噬细胞将微生物入侵者吞噬到质膜衍生的吞噬体中。这些成熟成杀微生物吞噬溶酶体，导致杀死摄入的微生物。吞噬体成熟涉及吞噬体与早期内体、晚期内体和细胞中的主要降解隔室溶酶体的顺序融合。一些细菌病原体操纵吞噬体膜的磷酸肌醇（PIP）组成，并且不被递送至吞噬溶酶体，这表明PIP在吞噬体成熟中的作用。这一假设得到了全面的微观研究的支持。最近，吞噬体和内（溶酶体）体之间融合的无细胞重建鉴定了磷脂酰肌醇4-磷酸[PI（4）P]和磷脂酰肌醇3-磷酸[PI（3）P]作为吞噬溶酶体生物发生的关键调节剂。在这里，我们描述了PIP在吞噬体成熟中的新兴作用，我们提出了使用完整细胞或纯化隔室研究PIP参与吞噬体运输的工具。
Professional phagocytes engulf microbial invaders into plasma membrane-derived phagosomes. These mature into microbicidal phagolysosomes, leading to killing of the ingested microbe. Phagosome maturation involves sequential fusion of the phagosome with early endosomes, late endosomes, and the main degradative compartments in cells, lysosomes. Some bacterial pathogens manipulate the phosphoinositide (PIP) composition of phagosome membranes and are not delivered to phagolysosomes, pointing at a role of PIPs in phagosome maturation. This hypothesis is supported by comprehensive microscopic studies. Recently, cell-free reconstitution of fusion between phagosomes and endo(lyso)somes identified phosphatidylinositol 4-phosphate [PI(4)P] and phosphatidylinositol 3-phosphate [PI(3)P] as key regulators of phagolysosome biogenesis. Here, we describe the emerging roles of PIPs in phagosome maturation and we present tools to study PIP involvement in phagosome trafficking using intact cells or purified compartments.