Acetylcholine receptor synthesis in retina and transport to optic tectum in goldfish.
Acetylcholine receptor synthesis in retina and transport to optic tectum in goldfish.
复制标题
金鱼视网膜中乙酰胆碱受体的合成和视顶盖的转运。
DOI：
10.1126/science.3715468
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Oswald,RE
中科院分区：
文献类型：
--
作者：
Henley,JM;Lindstrom,JM;Oswald,RE
先前的研究表明，金鱼的视网膜顶盖系统含有对α-银环蛇毒素敏感的烟碱乙酰胆碱受体（nAChR）。α-银环蛇毒素可以阻断视觉刺激引起的细胞外记录场电位，并且α-银环蛇毒素可以干扰视网膜顶盖突触连接的维持。视网膜神经节细胞和顶盖细胞之间的传输是否是由乙酰胆碱介导的，以及顶盖细胞树突上是否存在nAChR仍是一个问题。本报告中描述的实验旨在确定与金鱼视网膜顶盖投射相关的nAChR合成的位点。放射性（35 S标记）甲硫氨酸被注射到眼睛或顶盖脑室，并纳入nAChR的放射性通过免疫沉淀法测定。该技术的使用提供了证据表明，与金鱼retinotectal投影相关的nAChR在视网膜中合成并运输到视顶盖，这表明乙酰胆碱作用于视网膜末梢的突触前位点。
Previous studies have suggested that the retinotectal system of the goldfish contains a nicotinic acetylcholine receptor (nAChR) that is sensitive to α-bungarotoxin. Extracellularly recorded field potentials elicited in response to visual stimulation can be blocked by α-bungarotoxin, and α-bungarotoxin can interfere with the maintenance of retinotectal synaptic connections. Whether the transmission between the retinal ganglion cells and the tectal cells is mediated by acetylcholine and whether nAChR's exist on the dendrites of tectal cells are questions that remain. The experiments described in this report were designed to determine the site of synthesis of the nAChR's associated with the goldfish retinotectal projection. Radioactive (35S-labeled) methionine was injected into either the eye or the tectal ventricle, and the incorporation of radioactivity into the nAChR was measured by immunoprecipitation. The use of this technique provides evidence that an nAChR associated with the goldfish retinotectal projection is synthesized in the retina and transported to the optic tectum, which suggests a presynaptic site of acetylcholine action on retinal terminals.