Proliferation kinetics of perturbed cell populations determined by the bromodeoxyuridine-33258 technique: radiotoxic effects of incorporated [3H]thymidine.
Proliferation kinetics of perturbed cell populations determined by the bromodeoxyuridine-33258 technique: radiotoxic effects of incorporated [3H]thymidine.
复制标题
通过 bromodeoxyuridine-33258 技术测定的扰动细胞群的增殖动力学：掺入的 [3H]胸苷的放射毒性作用。
DOI：
10.1002/cyto.990020307
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
H. Beck
中科院分区：
文献类型：
--
作者：
H. Beck
描述了一种简单的分析方法，用于评价通过应用BrdUrd-33258 Hoechst技术获得的扰动细胞群体的流式细胞术数据。该程序允许确定生长曲线、从G2 + M流出和流入G2 + M以及细胞群通过G2 + M期的转运特征。作为该方法应用的一个例子，描述了掺入的~ 3 H-胸苷对L-929细胞体外增殖的放射毒性作用。
A simple analytical method is described for the evaluation of flow cytometric data of perturbed cell populations obtained by applying the BrdUrd-33258 Hoechst technique. This procedure allows for determining the growth curve, the efflux from and the influx into G2 + M as well as the transit characteristics of cell cohorts through the G2 + M phase. As an example of the application of the method, the radiotoxic effects of incorporated 3H-thymidine on the proliferation of L-929 cells in vitro are described.