Co-localization of major quantitative trait loci for pod size and weight to a 3.7 cM interval on chromosome A05 in cultivated peanut (Arachis hypogaea L.).
Co-localization of major quantitative trait loci for pod size and weight to a 3.7 cM interval on chromosome A05 in cultivated peanut (Arachis hypogaea L.).
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栽培花生 (Arachis hypergaea L.) 荚果大小和重量主要数量性状基因座在 A05 染色体上 3.7 cM 间隔的共定位
DOI：
10.1186/s12864-016-3456-x
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发表时间：
2017-01-09
期刊：
影响因子：
4.4
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Luo H;Ren X;Li Z;Xu Z;Li X;Huang L;Zhou X;Chen Y;Chen W;Lei Y;Liao B;Pandey MK;Varshney RK;Guo B;Jiang X;Liu F;Jiang H
栽培花生（Arachis hypogaea L.），是食用油和蛋白质的重要来源，广泛种植于世界热带和亚热带地区。产量相关性状的遗传改良是提高花生新品种产量潜力的关键。基因组辅助育种（GAB）可以加速遗传改良过程，但需要相关性状的连锁标记。在此背景下，构建了一个由195个单株组成的重组自交系（RIL）作图群体（元杂9102 ×徐州68-4），并利用该群体定位了与3个重要角果性状（角果长、角果宽和百角果重）相关的数量性状基因座（QTL）。QTL分析使用的表型数据产生的4个环境中在两个地点和743个定位位点的基因型数据确定了15个QTL的荚长，11个QTL的荚宽和16个QTL的百荚重。解释的表型变异（PVE）范围为3.68%至27.84%。13个QTL在至少2个环境中稳定表达，3个QTL（qPLA05.7、qPLA09.3和qHPWA05.6）在4个环境中稳定表达。在A05染色体上共定位了3个主效QTL，分别为：控制荚长的qPLA05.7（16.89- 27.84%PVE）、控制荚宽的qPWA05.5（13.73- 14.12%PVE）和控制百荚重的qHPWA05.6（13.75- 26.82%PVE）。该3.7cM的连锁区间对应于A. duranensis的基因序列，包括114个与催化活性和代谢过程相关的基因。本研究共定位了3个控制角果大小和角果重量的QTL，它们位于A05染色体上3.7cM的区间内。这些QTL区域不仅为基因发现和功能标记的开发提供了进一步的研究，而且为在GAB中利用这些QTL提高花生产量提供了机会。
Cultivated peanut (Arachis hypogaea L.), an important source of edible oil and protein, is widely grown in tropical and subtropical areas of the world. Genetic improvement of yield-related traits is essential for improving yield potential of new peanut varieties. Genomics-assisted breeding (GAB) can accelerate the process of genetic improvement but requires linked markers for the traits of interest. In this context, we developed a recombinant inbred line (RIL) mapping population (Yuanza 9102 × Xuzhou 68-4) with 195 individuals and used to map quantitative trait loci (QTLs) associated with three important pod features, namely pod length, pod width and hundred-pod weight. QTL analysis using the phenotyping data generated across four environments in two locations and genotyping data on 743 mapped loci identified 15 QTLs for pod length, 11 QTLs for pod width and 16 QTLs for hundred-pod weight. The phenotypic variation explained (PVE) ranged from 3.68 to 27.84%. Thirteen QTLs were consistently detected in at least two environments and three QTLs (qPLA05.7, qPLA09.3 and qHPWA05.6) were detected in all four environments indicating their consistent and stable expression. Three major QTLs, detected in at least three environments, were found to be co-localized to a 3.7 cM interval on chromosome A05, and they were qPLA05.7 for pod length (16.89–27.84% PVE), qPWA05.5 for pod width (13.73–14.12% PVE), and qHPWA05.6 for hundred-pod weight (13.75–26.82% PVE). This 3.7 cM linkage interval corresponds to ~2.47 Mb genomic region of the pseudomolecule A05 of A. duranensis, including 114 annotated genes related to catalytic activity and metabolic process. This study identified three major consistent and stable QTLs for pod size and weight which were co-localized in a 3.7 cM interval on chromosome A05. These QTL regions not only offer further investigation for gene discovery and development of functional markers but also provide opportunity for deployment of these QTLs in GAB for improving yield in peanut.