Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR.
Creatine utilization as a sole nitrogen source in Pseudomonas putida KT2440 is transcriptionally regulated by CahR.
复制标题
恶臭假单胞菌 KT2440 中肌酸作为唯一氮源的利用受到 CahR 的转录调控。
DOI：
10.1099/mic.0.001145
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Wargo,MatthewJ
中科院分区：
文献类型：
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作者：
Hinkel,LaurenA;Willsey,GrahamG;Lenahan,SeanM;Eckstrom,Korin;Schutz,KristinC;Wargo,MatthewJ
含有谷氨酰胺转移酶-1结构域的AraC家族转录调节因子（GATR）存在于许多细菌的基因组中，包括所有假单胞菌。已经确定了几种特征性GATR参与含胺化合物代谢，但GATR配体和调控网络的全部范围仍然未知。在这里，我们描述了恶臭假单胞菌对动物源性胺化合物肌酸的检测，肌酸是一种通过肌酸特异性GATR在肌肉和纤毛细胞中特别富集的化合物，PP_3665，在这里被命名为CahR（CreatineamidohydrolaseRegulator）。putida利用肌酸作为唯一氮源的能力。CahR/肌酸调节子很小，电泳迁移率变动分析表明，仅在thecreA启动子处具有强而特异的CahR结合，支持大部分调节子依赖于下游代谢物的结论。与cahR同源物相关的creA同源物的系统发育分析突出了支持可能的水平基因转移的菌株分布和组织，特别是在不动杆菌属中。本研究鉴定并表征了转录控制P. putida的肌酸代谢，拓宽了已知的GATR配体的范围，并建议在脂肪族氮化合物代谢的演变过程中GATR多样化。
Glutamine amidotransferase-1 domain-containing AraC-family transcriptional regulators (GATRs) are present in the genomes of many bacteria, including allPseudomonasspecies. The involvement of several characterized GATRs in amine-containing compound metabolism has been determined, but the full scope of GATR ligands and regulatory networks are still unknown. Here, we characterizePseudomonas putida’s detection of the animal-derived amine compound creatine, a compound particularly enriched in muscle and ciliated cells by a creatine-specific GATR, PP_3665, here named CahR (CreatineamidohydrolaseRegulator).cahRis necessary for transcription of the gene encoding creatinase (PP_3667/creA) in the presence of creatine and is critical forP. putida’s ability to utilize creatine as a sole source of nitrogen. The CahR/creatine regulon is small, and an electrophoretic mobility shift assay demonstrates strong and specific CahR binding only at thecreApromoter, supporting the conclusion that much of the regulon is dependent on downstream metabolites. Phylogenetic analysis ofcreAorthologues associated withcahRorthologues highlights a strain distribution and organization supporting probable horizontal gene transfer, particularly evident within the genusAcinetobacter. This study identifies and characterizes the GATR that transcriptionally controlsP. putida’s metabolism of creatine, broadening the scope of known GATR ligands and suggesting GATR diversification during evolution of metabolism for aliphatic nitrogen compounds.