Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity.
Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity.
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DOI：
10.1016/s1001-0742(09)60109-1
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发表时间：
2010
影响因子：
6.9
通讯作者：
Taowu Ma;S. Gong;Ke Zhou;Cheng Zhu;Kaidong Deng;Q. Luo;Zijian Wang
中科院分区：
文献类型：
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作者：
Taowu Ma;S. Gong;Ke Zhou;Cheng Zhu;Kaidong Deng;Q. Luo;Zijian Wang
本研究旨在为淡水腹足动物铜绿贝拉米亚（Reeve）开发原始的实验室培养和沉积物毒性测试方案，这是一种用于沉积物毒性测试的新潜在物种。在建议的实验室条件下，使用有效的培养系统成功培养了铜绿假单胞菌。幼虫、幼虫和成虫的最佳随意摄食水平分别为2.0、6.0 和16.0 毫克鱼食/（蜗牛·天）。幼鱼平均存活率高于90%。蜗牛在9周龄时即可确定性别，其世代时间约为4个月。繁殖全年持续；平均生育力为0.55个新生儿/（雌性·天）。在人工沉积物为期 10 天和 28 天的案例研究中评估了该物种的生物测定效用。 Cu 对幼虫的 10 天 LC50 为 480 μg/g 干重 (dw)，观察到的 Cu 对幼虫存活和生长的最低影响浓度为 195 μg/g dw。存活和生长是急性毒性的可靠指标。幼虫比成虫积累更多的铜。铜绿假单胞菌表现出比标准测试物种（Hyalella azteca 和 Chironomus tentans）更高的对铜暴露的敏感性。对成虫进行的为期 28 天的沉积物毒性试验表明，繁殖力是生殖毒性的一个强有力的终点指标，超氧化物歧化酶、过氧化氢酶和谷胱甘肽的生化终点可作为铜诱导的氧化损伤的敏感生物标志物。因此，可以推荐铜绿假单胞菌作为淡水沉积物毒性测试方案标准化的候选者。
This study aimed to develop original laboratory culture and sediment toxicity testing protocols for the freshwater gastropod Bellamya aeruginosa (Reeve), a new potential species for sediment toxicity testing. B. aeruginosa was successfully cultured with an effective culture system under proposed laboratory conditions. Optimal ad libitum feeding levels for larvae, juveniles, and adults were 2.0, 6.0, and 16.0 mg fish food/(snail·day), respectively. Mean survival rates of juveniles were higher than 90%. The snails could be sexed at 9 weeks of age, and their generation time is approximately 4 months. Reproduction continued all year around; the mean fecundity was 0.55 newborn/(female·day). The utility of this species for bioassays was evaluated in both 10-day and 28-day case studies with artificial sediments. The 10-day LC50of Cu for larvae was 480 μg/g dry weight (dw), and the lowest observed effects concentration of Cu for survival and growth of larvae was 195 μg/g dw. Survival and growth are reliable indicators of acute toxicity. Larvae accumulated more Cu than adults. B. aeruginosa exhibited a higher sensitivity to Cu exposure than standard test species (Hyalella azteca and Chironomus tentans). The 28-day test of sediment toxicity with adults showed that fecundity was a robust endpoint indicator of reproductive toxicity, and the biochemical endpoints of superoxide dismutase, catalase, and glutathione could be used as sensitive biomarkers for Cu-induced oxidative damage. B. aeruginosa can be therefore recommended as a candidate for the standardization of the freshwater sediment toxicity test protocol.