Effects of blood anticoagulants on stable isotope values of sea turtle blood tissue
Effects of blood anticoagulants on stable isotope values of sea turtle blood tissue
复制标题
血液抗凝剂对海龟血液组织稳定同位素值的影响
DOI：
10.3354/ab00397
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发表时间：
2012
期刊：
影响因子：
1.4
通讯作者：
J. Seminoff
中科院分区：
文献类型：
--
作者：
G. E. Lemons;Tomoharu Eguchi;Boyd N. Lyon;R. LeRoux;J. Seminoff
从海龟身上采集组织样本进行稳定同位素分析经常发生在偏远的野外。对于血液组织，用抗凝剂处理样本，以便随后将血浆与细胞成分分离。然而，该技术对海龟血液稳定同位素值的影响尚未确定。我们测量了3种广泛使用的抗凝剂，酸性柠檬酸葡萄糖（ACD），肝素钠（SH）和乙二胺四乙酸（EDTA）对稳定碳（！13 C）和稳定氮（！在美国加州圣地亚哥湾捕获的11只绿色海龟的全血、红细胞和血浆中的15 N）值。以随机顺序灌装含有3种血液防腐剂的小瓶以及不含添加剂的小瓶（即对照小瓶）。通过离心将无添加剂小瓶中的血液立即分离成组分（例如红细胞、血浆），而将处理小瓶中收集的血液冷却，然后在收集后48小时离心。我们发现，相对于对照组，ACD保存的全血和血浆富集13 C，EDTA处理的红细胞和血浆缺乏15 N，SH处理的全血富集15 N。由于SH是唯一一种对血浆和红细胞（海龟稳定同位素研究中最常研究的血液组分）没有测量影响的抗凝剂，因此我们建议将其专门用作无法快速离心的现场研究的血液抗凝剂。
Collecting tissue samples from sea tur- tles for stable isotope analysis often occurs at remote field sites. For blood tissue, samples are treated with an anticoagulant that allows for later separation of plasma from cellular components. However, the effect of this technique on stable isotope values of sea turtle blood has not been established. We measured the effects of 3 widely used anticoagulants, acid citrate dextrose (ACD), sodium heparin (SH) and ethylenediaminetetraacetic acid (EDTA), on stable carbon (! 13 C) and stable nitrogen (! 15 N) values in whole blood, red blood cells, and blood plasma of 11 green turtles Chelonia mydas captured in San Diego Bay, California, USA. Vials containing each of the 3 blood preservatives as well as a vial containing no additive (i.e. control vial) were filled in random order. Blood in the no-additive vial was immediately sepa- rated into fractions (e.g. red blood cells, plasma) via centrifugation, whereas blood collected in the treat- ment vials was chilled and then centrifuged 48 h after collection. We found that, relative to the con- trols, ACD-preserved whole blood and blood plasma were 13 C enriched, EDTA-treated red blood cells and plasma were 15 N de pleted, and SH-treated whole blood was 15 N en riched. Because SH was the only anticoagulant with no measured effect on blood plasma and red blood cells — the most commonly studied blood fractions for sea turtle stable isotope studies — we recommend its exclusive use as a blood anticoagulant for field studies where prompt centri - fugation is not possible.
影响因子：
20.3
作者：
Rand, MD;Lock, JB;Mann, KG
通讯作者：
Mann, KG