Optimization of the tissue source, malignancy, and initial substrate of tumor cell-derived matrices to increase cancer cell chemoresistance against 5-fluorouracil.
Optimization of the tissue source, malignancy, and initial substrate of tumor cell-derived matrices to increase cancer cell chemoresistance against 5-fluorouracil.
复制标题
优化肿瘤细胞衍生基质的组织来源、恶性肿瘤和初始底物，以增加癌细胞对 5-氟尿嘧啶的化疗耐药性。
DOI：
10.1016/j.bbrc.2014.12.116
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M. Tanaka
中科院分区：
文献类型：
--
作者：
T. Hoshiba;M. Tanaka
体外肿瘤细胞的低耐药性抑制了新抗癌药物的开发。因此，需要开发新的体外培养系统来增加体外癌细胞的化学抗性。肿瘤细胞来源的基质已被报道增加体外癌细胞的化疗耐药性。然而，目前尚不清楚组织来源和用于制备基质的细胞的恶性程度如何影响肿瘤细胞衍生基质的耐药性。此外，目前还不清楚用于制备基质的初始底物如何影响化学抗性。在这项研究中，我们比较了肿瘤细胞的组织来源和恶性程度的影响，以及对5-氟尿嘧啶（5-FU）的化疗耐药的初始底物的影响。乳腺癌和结肠癌细胞对5-FU的耐药性在用来自具有较高恶性度的相应原始组织的细胞制备的基质上增加。此外，对5-FU的耐药性改变了使用不同的初始底物，表现出不同的蛋白质吸附特性的矩阵。总之，这些结果表明，适当选择组织来源、肿瘤细胞的恶性程度和用于基质制备的初始底物对于制备肿瘤细胞衍生基质进行化疗耐药性试验非常重要。
The low chemoresistance ofin vitrocancer cells inhibits the development of new anti-cancer drugs. Thus, development of a newin vitroculture system is required to increase the chemoresistance ofin vitrocancer cells. Tumor cell-derived matrices have been reported to increase the chemoresistance ofin vitrocancer cells. However, it remains unclear how tissue sources and the malignancy of cells used for the preparation of matrices affect the chemoresistance of tumor cell-derived matrices. Moreover, it remains unclear how the initial substrates used for the preparation of matrices affect the chemoresistance. In this study, we compared the effects of tissue sources and the malignancy of tumor cells, as well as the effect of the initial substrates on chemoresistance against 5-fluorouracil (5-FU). The chemoresistance of breast and colon cancer cells against 5-FU increased on matrices prepared with cells derived from the corresponding original tissues with higher malignancy. Moreover, the chemoresistance against 5-FU was altered on matrices prepared using different initial substrates that exhibited different characteristics of protein adsorption. Taken together, these results indicated that the appropriate selection of tissue sources, malignancy of tumor cells, and initial substrates used for matrix preparation is important for the preparation of tumor cell-derived matrices for chemoresistance assays.