Smart "Turn-on" magnetic resonance contrast agents based on aptamer-functionalized superparamagnetic iron oxide nanoparticles
Smart "Turn-on" magnetic resonance contrast agents based on aptamer-functionalized superparamagnetic iron oxide nanoparticles
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DOI：
10.1002/cbic.200700323
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发表时间：
2007-09-24
期刊：
影响因子：
3.2
通讯作者：
Lu, Yi
中科院分区：
文献类型：
--
作者：
Yigit, Mehmet Veysel;Mazumdar, Debapriya;Lu, Yi
适体是单链DNA或RNA分子，可以以高亲和力和选择性结合各种化学和生物分子。[1-4]它们是通过使用称为指数富集配体系统进化（SELEX）的组合生物学技术从大量随机DNA或RNA分子中分离出来的。[1，2]它们在与广泛分子的选择性和敏感性结合方面通常与抗体相当。[5-8]这些分子相对于抗体的主要优势在于它们可以相对容易地被选择用于任何目标分析物以及它们对生物降解和变性的稳定性。由于这些性质，适体是用于制造许多领域中的化学和生物传感器的良好候选者，例如医学诊断和环境监测。因此，适体已被转化为荧光、[9-22]比色、[23-29]和电化学传感器。[30-33]虽然适体传感器已经在体外进行了广泛的探索，但它们在体内的应用，特别是在hu-ACHTUNGTRENNUNG人中的应用，仍然是一个重大的挑战，因为光难以穿透皮肤和来自细胞组分的信号干扰。
Aptamers are single-stranded DNA or RNA molecules that can bind a variety of chemical and biological molecules with high affinity and selectivity.[1–4] They are isolated from a large random pool of DNA or RNA molecules by using a combinatorial-biology technique called systematic evolution of ligands by exponential enrichment (SELEX).[1, 2] They are often comparable to antibodies in their selective and sensitive binding to a broad range of molecules.[5–8] The major advantage of these molecules over antibodies lies in the relative ease with which they can be selected for any target analyte and their stability against biodegradation and denaturation. Due to these properties aptamers are good candidates for making chemical and biological sensors in many fields, such as medical diagnostics and environmental monitoring. Therefore, aptamers have been converted into fluorescent,[9–22] colorimetric,[23–29] and electrochemical sensors.[30–33] While aptamer sensors have been wideACHTUNGTRENNUNGly explored in vitro, their application in vivo, particularly in hu-ACHTUNGTRENNUNGmans, remains a significant challenge because of the difficulty in light penetration through the skin and signal interference from cellular components.