Water channel-carrying vesicles in the rat IMCD contain cellubrevin.
Water channel-carrying vesicles in the rat IMCD contain cellubrevin.
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大鼠 IMCD 中携带水通道的囊泡含有纤维蛋白。
DOI：
10.1152/ajpcell.1995.269.3.c797
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Hays,RM
中科院分区：
文献类型：
--
作者：
Franki,N;Macaluso,F;Schubert,W;Gunther,L;Hays,RM
抗利尿激素（精氨酸加压素）诱导集合管中水通道囊泡的对接、融合和内吞的循环过程。现在有证据表明，对接和内吞作用是由一系列与囊泡和靶膜相关的蛋白质介导的。在最近的研究中，我们已经表明，cellubrevin，囊泡相关膜蛋白家族的成员，以及其他对接蛋白，在大鼠内髓集合管中表达。现在，我们通过免疫金电子显微镜，cellubrevin是存在于囊泡含有水通道，它是与涂层和未涂层囊泡，它是存在于顶膜。因此，Cellubrevin能够介导精氨酸加压素诱导的水通道循环中的一个或多个步骤。
Antidiuretic hormone (arginine vasopressin) induces a cyclic process of docking, fusion, and endocytosis of water channel-containing vesicles in the collecting duct. There is now evidence that docking and endocytosis are mediated by an array of proteins associated with vesicles and target membranes. In recent studies, we have shown that cellubrevin, a member of the vesicle-associated membrane protein family, as well as other docking proteins, are expressed in the rat inner medullary collecting duct. We now show by immunogold electron microscopy that cellubrevin is present on vesicles containing water channels, that it is associated with both coated and uncoated vesicles, and that it is present on the apical membrane. Cellubrevin, therefore, is in a position to mediate one or more steps in arginine vasopressin-induced water channel cycling.