MOLECULAR-CLONING AND EXPRESSION OF A G25K CDNA, THE HUMAN HOMOLOG OF THE YEAST-CELL CYCLE GENE CDC42
MOLECULAR-CLONING AND EXPRESSION OF A G25K CDNA, THE HUMAN HOMOLOG OF THE YEAST-CELL CYCLE GENE CDC42
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DOI：
10.1128/mcb.10.11.5977
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发表时间：
1990-11-01
影响因子：
5.3
通讯作者：
POLAKIS, P
中科院分区：
文献类型：
--
作者：
MUNEMITSU, S;INNIS, MA;POLAKIS, P
G25K是一种低分子质量的GTP结合蛋白，在哺乳动物组织中分布广泛。利用与纯化的人G25K部分氨基酸序列相对应的寡核苷酸序列，克隆了一个人G25K的cDNA克隆。该基因编码191个氨基酸的多肽，含有与GTP结合的共同序列，在C末端有一个可能的法尼化位点。该序列与哺乳动物Rho和Rac蛋白的同源性分别为50%和70%，与酿酒酵母CDc42基因产物的同源性为80%。用表达G25K基因的重组杆状病毒载体感染昆虫Sf9细胞，可产生一种结合GTP的25 kDa蛋白，并可被G25K特异性抗体识别。G25K似乎是cdc42基因产物的人类同源物，因为G25K在酿酒酵母中的表达抑制了cdc42-1和cdc24-4温度敏感的致命突变。
G25K is a low-molecular-mass GTP-binding protein with a broad distribution in mammalian tissues. A cDNA clone was isolated by using oligonucleotides corresponding to the partial amino acid sequence of purified human G25K. The cDNA encodes an 191-amino-acid polypeptide containing GTP-binding consensus sequences and a putative farnesylation site at the C terminus. The sequence exhibits 50 and 70% identities to the mammalian rho and rac proteins, respectively, and an 80% identity to the Saccharomyces cerevisiae CDC42 gene product. Insect Sf9 cells infected with recombinant baculovirus vectors expressing the G25K cDNA produced a 25-kDa protein that bound GTP and was recognized by antibodies specifically reactive to G25K. G25K appears to be the human homolog of the CDC42 gene product, since expression of the G25K cDNA in S. cerevisiae suppressed both cdc42-1 and cdc24-4 temperature-sensitive lethal mutations.