ΔpH‐dependent chlorophyll fluorescence quenching indicating a mechanism of protection against photoinhibition of chloroplasts
ΔpH‐dependent chlorophyll fluorescence quenching indicating a mechanism of protection against photoinhibition of chloroplasts
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ΔpH 依赖性叶绿素荧光猝灭表明叶绿体免受光抑制的保护机制
DOI：
10.1016/0014-5793(86)81156-5
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发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
U. Behrend
中科院分区：
文献类型：
--
作者：
G. Krause;U. Behrend
完整的分离的菠菜叶绿体进行光抑制条件（高光和缺乏CO2）。当叶绿体处于低荧光状态时，电子传递系统的光抑制显著减弱，这与类囊体膜上的高质子梯度有关，而在高荧光状态下，通过加入解偶联剂消除了Δ pH依赖的荧光猝灭。假设进行了讨论，在叶绿体暴露于过量的光，光抑制部分地防止由增加的热耗散的激发能，所表示的Δ pH值依赖（“能量依赖”）叶绿素荧光猝灭。
Intact isolated spinach chloroplasts were subjected to photoinhibitory conditions (high light and lack of CO2). Photoinhibition of the electron transport system was considerably diminished when the chloroplasts were in a low-fluorescent state related to a high proton gradient across the thylakoid membranes, as compared to a high-fluorescent state in which ΔpH-dependent fluorescence quenching was abolished by addition of uncouplers. The hypothesis is discussed that in chloroplasts exposed to excess light, photoinhibition is partly prevented by increased thermal dissipation of excitation energy, as expressed by ΔpH-dependent (‘energy-dependent’) chlorophyllafluorescence quenching.