Comparison of conventional ELISA with electrochemiluminescence technology for detection of amyloid-β in plasma.
Comparison of conventional ELISA with electrochemiluminescence technology for detection of amyloid-β in plasma.
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DOI：
10.3233/jad-2010-100456
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Mehta PD
中科院分区：
文献类型：
--
作者：
Oh ES;Mielke MM;Rosenberg PB;Jain A;Fedarko NS;Lyketsos CG;Mehta PD
血浆β淀粉样蛋白（Aβ）水平可作为阿尔茨海默病研究的一个非侵入性生物标志物。我们比较了多重电化学发光检测方法和已建立的ELISA方法用于血浆Aβ定量。与ELISA方法相比，电化学发光检测方法显示出统计学显著性，但适度相关。其原因可能包括抗体亲和力的差异以及用作标准品的Aβ肽的纯度和来源。但是电化学发光检测技术的优点包括处理时间短和样品体积小。这种比较表明，需要进一步研究，优化该系统。
Plasma amyloid-β (Aβ) level could be useful as a non-invasive biomarker in Alzheimer’s disease research. We compared a multiplex electrochemiluminescence detection method with a well established ELISA method for plasma Aβ quantification. Compared to the ELISA method, the electrochemiluminescence detection method demonstrates a statistically significant, but modest correlation. The reasons for this may include the differences in the affinities of antibodies, and purity and source of Aβ peptides used as standards. However, the advantages of electrochemiluminescence detection technology include short processing time and small sample volume. This comparison demonstrates the need for a further study in optimizing this system.