Coining attributes of ultra-low concentration graphene oxide and spermine: An approach for high strength, anti-microbial and osteoconductive nanohybrid scaffold for bone tissue regeneration
Coining attributes of ultra-low concentration graphene oxide and spermine: An approach for high strength, anti-microbial and osteoconductive nanohybrid scaffold for bone tissue regeneration
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DOI：
10.1016/j.carbon.2018.09.062
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发表时间：
2019-01-01
期刊：
影响因子：
10.9
通讯作者：
Chattopadhyay, Santanu
中科院分区：
文献类型：
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作者：
Ghorai, Sanjoy Kumar;Maji, Somnath;Chattopadhyay, Santanu
严重创伤后缺乏自体成骨和骨修复的临床复杂性，迫切需要开发一种重要的方法来促进骨的再生。本工作研究了二维棒状纳米羟基磷灰石(NHA)在超低浓度氧化石墨烯(GO)表面的修饰。采用原位技术将纳米杂化材料(GO-NHA)引入到基于精胺的高强度热塑性聚氨酯-尿素(PUU)基质中，制备了多孔支架。1wt%的GO-nHA填充支架的物理力学性能显著提高。用类成骨细胞系MG-63进行的细胞毒性实验显示，在14天的培养期间，细胞存活率为95%以上，并促进了细胞的增殖。半定量实时聚合酶链式反应(qRT-PCR)显示I型胶原和骨钙素呈阳性表达，表明成骨细胞有良好的成熟和生物矿化。为了避免体内碳质颗粒的负担，在支架中加入了很低比例的GO(0.15%)，以显著提高力学性能、表面润湿性、细胞存活率和细胞增殖能力。此外，体内实验证实PUU/GO-nHA支架在体内具有促进成骨作用，且无任何细胞毒性。基于力学、体外和体内研究，高强度纳米杂化支架在骨科应用方面显示出巨大的潜力。(C)2018爱思唯尔有限公司。保留所有权利。
Lack of auto osteogenesis and clinical complexities of repairing of bone after severe injuries, urge to develop a significant approach to promote regeneration of bone. The present work represents ornamentation of 2D rod-like nanohydroxyapatite (nHA) on ultra-low concentration graphene oxide (GO) sheet. The nanohybrids (GO-nHA) were incorporated into the spermine based high strength thermoplastic polyurethane-urea (PUU) matrices by in situ technique and the porous scaffolds were fabricated. 1 wt% GO-nHA filled scaffold displayed dramatically improved physico-mechanical properties. Cytotoxicity study using osteoblast cell like MG-63 cell line revealed positive cell viability (above 95%) and increased proliferation over a period of 14 days of culture. Semi-quantitative Real Time polymerase Chain Reaction (qRT-PCR) showed positive expression of collagen type I and osteocalcin indicating excellent maturation and biomineralization of osteoblasts. To avoid burden of carbonaceous particulate in body, very low percentage of GO (0.15%) was incorporated into the scaffold to improve mechanical properties, surface wettability, cell viability and cell proliferation drastically. Furthermore, in vivo study confirmed the promoted osteogenesis of PUU/GO-nHA scaffold in vivo without any cytotoxicity. Based on mechanical, in vitro and in vivo study the high strength nanohybrid scaffold exhibited tremendous potential for orthopedic applications. (C) 2018 Elsevier Ltd. All rights reserved.