Long-term Effect of Ubiquinol on Exercise Capacity and the Oxidative Stress Regulation System in SAMP1 Mice
Long-term Effect of Ubiquinol on Exercise Capacity and the Oxidative Stress Regulation System in SAMP1 Mice
复制标题
泛醇对 SAMP1 小鼠运动能力和氧化应激调节系统的长期影响
DOI：
--
复制
发表时间：
2014
影响因子：
--
通讯作者：
S. Kido
中科院分区：
文献类型：
--
作者：
H. Maruoka;K. Fujii;Kazuhisa Inoue;S. Kido
[目的]研究长期摄入不同剂量的泛醌醇（还原型辅酶Q10，H2 CoQ 10：QH）对运动能力和氧化应激调节系统的影响。[对象与方法] 23只快速衰老小鼠P1（SAMP 1）随机分为两组：一组摄入相对高量的QH（300 mg/kg; A组），另一组摄入相对低量的QH（30 mg/kg，B组）。 自由进食和饮用自来水。两组都在跑步机上跑步直到筋疲力尽，并测量总跑步时间。对于氧化应激调节系统，在静息状态下测量d-ROM测试值（氧化应激程度）和BAP测试值（抗氧化潜力），然后计算BAP/d-ROM比率（B/R比率）。还测量了血浆QH和血浆泛醌（辅酶Q10的血浆氧化形式）的值，并计算了还原率。进行3次测量：在研究开始时，动物为39周龄（基线），食用QH 7个月（7 mo）后，以及食用QH 10个月（10 mo）后。[结果] 10 mo时A组衰老评分明显低于B组。运行时间平均百分比变化的比较显示，两组之间的差异为15.1%。10个月时，B组的d-ROM测试值显著增加，B/R比值显著降低。在A组中观察到血浆QH值显著升高和比值降低。[结论]与B组相比，A组的d-ROM测试下降幅度更大，下降率更高。因此，证明了QH消耗的剂量依赖性效应。
[Purpose] This study examined how exercise capacity and the oxidative stress regulation system are affected by different amounts of dietary Ubiquinol (reduced form of coenzyme Q10, H2CoQ10: QH) over the long term. [Subjects and Methods] Twenty-three senescence-accelerated mouse P1 (SAMP1) mice were randomly divided into two groups: one consuming a relatively high amount of QH (300 mg/kg; Group A) and the other a relatively low amount (30 mg/kg, Group B). Food and tap water were provided ad libitum. Both groups were made to run on a treadmill until exhaustion, and total running duration was measured. For the oxidative stress regulation system, the d-ROM test value (degree of oxidative stress) and BAP test value (antioxidant potential) were measured in a resting state, and then the BAP/d-ROM ratio (B/R ratio) was calculated. The values of plasma QH and plasma ubiquinone (plasma oxidized form of CoQ10) were also measured, and the reduced ratio was calculated. Measurements were taken 3 times: at the start of the study when the animals were 39 weeks old (baseline), after consumption of QH for 7 months (7 mo), and after consumption of QH for 10 months (10 mo). [Results] The senescence score at 10 mo was significantly lower in Group A. Comparison of the mean percentage change in running time showed a difference of 15.1% between the 2 groups. At 10 mo, the d-ROM test value was significantly increased and the B/R ratio was significantly decreased in Group B. Significant increases in the plasma QH value and reduced ratio were seen in Group A. [Conclusion] Group A showed a greater decrease in the d-ROM test and increase in the reduced ratio than Group B. Thus, a dose-dependent effect of QH consumption was demonstrated.
影响因子：
3.3
作者：
Jiankang Liu;H. C. Yeo;Eva Övervik-Douki;T. Hagen;S. Doniger;Daniel W. Chu;George A. Brooks;B. Ames
通讯作者：
Jiankang Liu;H. C. Yeo;Eva Övervik-Douki;T. Hagen;S. Doniger;Daniel W. Chu;George A. Brooks;B. Ames