Characterization and expression analysis of dirigent family genes related to stresses in Brassica
Characterization and expression analysis of dirigent family genes related to stresses in Brassica
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DOI：
10.1016/j.plaphy.2013.02.030
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发表时间：
2013-06-01
影响因子：
6.5
通讯作者：
Nou, Ill-Sup
中科院分区：
文献类型：
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作者：
Arasan, Senthil Kumar Thamil;Park, Jong-In;Nou, Ill-Sup
dirigent （DIR）基因在提高作物抗逆性方面起着至关重要的作用。在本研究中，我们收集了29个类似DIR的基因，其中2个来自一株油菜。从rapa数据库中获得了5个全长cDNA文库和27个BrDIR样基因。对这些基因进行序列分析和比对研究，证实7个基因为dirigent基因，其余22个基因为dirigent样基因。表达分析显示这些基因具有器官特异性表达。BrDIR2对尖孢镰刀菌有不同的反应。白菜粘菌病。与BrDIR2高度同源的4个甘蓝同源基因在感染该真菌的白菜植株中也表现出类似的反应。此外，几个BrDIR样基因在水分、ABA和冷胁迫处理后均表现出显著的响应。水分胁迫下，大多数响应基因在24 h表达量最高，此时相同胁迫条件下样品的酸溶木质素含量也最高，说明BrDIR类基因与木质素含量可能存在一定关系。综上所述，我们的研究结果表明BrDIR基因对芸苔属生物和非生物胁迫具有保护作用。(C) 2013 Elsevier Masson SAS。版权所有。
The dirigent (DIR) genes are playing a vital role in enhancing stress resistance in different crop plants. In this study, we collected 29 DIR like genes, two from a Brassica rapa cv. Osome full length cDNA library and 27 from the B. rapa database designated as B. rapa Dirigent (BrDIR) like genes. Sequence analysis and a comparison study of these genes confirmed that seven were dirigent and the remaining 22 were dirigent like genes. Expression analysis revealed an organ specific expression of these genes. BrDIR2 showed differential responses after Fusarium oxysporum f.sp. conglutinans infection in cabbage. Four Brassica oleracea dirigent like genes highly homologous to BrDIR2 also showed similar responses in cabbage plants infected with this fungus. Moreover, several BrDIR like genes showed significant responses after water, ABA and cold stress treatments in Chinese cabbage. Under water stress, most responsive genes showed the highest expression at 24 h, at which time the acid soluble lignin content of samples under the same stress condition were also highest, indicating a possible relationship between BrDIR like genes and lignin content. Taken together, our results indicate a protective role of BrDIR genes against biotic and abiotic stresses in Brassica. (C) 2013 Elsevier Masson SAS. All rights reserved.