Kunjin Virus, Zika Virus, and Yellow Fever Virus Infections Have Distinct Effects on the Coding Transcriptome and Proteome of Brain-Derived U87 Cells.
Kunjin Virus, Zika Virus, and Yellow Fever Virus Infections Have Distinct Effects on the Coding Transcriptome and Proteome of Brain-Derived U87 Cells.
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DOI：
10.3390/v15071419
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发表时间：
2023-06-23
期刊：
影响因子：
--
通讯作者：
Bisaillon M
中科院分区：
文献类型：
--
作者：
Brand C;Deschamps-Francoeur G;Bullard-Feibelman KM;Scott MS;Geiss BJ;Bisaillon M
作为专性的细胞内寄生虫，病毒严重依赖宿主细胞进行复制，因此为了自身的利益而扰乱了几个细胞过程。作为回报，宿主细胞激活多个信号通路，以限制病毒复制并根除病毒。本研究通过下一代RNA测序和质谱仪(SILAC)探索病毒和宿主细胞之间的复杂相互作用。将昆津病毒、寨卡病毒和黄热病病毒感染的人脑U87细胞的编码转录组和蛋白质组与模拟感染细胞的转录组和蛋白质组进行了比较。在每一次感染中都发现了数百个mRNA和蛋白质丰度的变化。此外，数百个mRNAs的选择性剪接被发现在病毒感染时受到调节。有趣的是，在感染细胞的转录组和蛋白质组的变化之间观察到了显著的脱节。这些发现提供了对黄病毒感染细胞的编码转录组和蛋白质组的全球视角，从而更好地理解了黄病毒与宿主的相互作用。
As obligate intracellular parasites, viruses rely heavily on host cells for replication, and therefore dysregulate several cellular processes for their benefit. In return, host cells activate multiple signaling pathways to limit viral replication and eradicate viruses. The present study explores the complex interplay between viruses and host cells through next generation RNA sequencing as well as mass spectrometry (SILAC). Both the coding transcriptome and the proteome of human brain-derived U87 cells infected with Kunjin virus, Zika virus, or Yellow Fever virus were compared to the transcriptome and the proteome of mock-infected cells. Changes in the abundance of several hundred mRNAs and proteins were found in each infection. Moreover, the alternative splicing of hundreds of mRNAs was found to be modulated upon viral infection. Interestingly, a significant disconnect between the changes in the transcriptome and those in the proteome of infected cells was observed. These findings provide a global view of the coding transcriptome and the proteome of Flavivirus-infected cells, leading to a better comprehension of Flavivirus–host interactions.
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影响因子：
6.7
作者：
De Maio FA;Risso G;Iglesias NG;Shah P;Pozzi B;Gebhard LG;Mammi P;Mancini E;Yanovsky MJ;Andino R;Krogan N;Srebrow A;Gamarnik AV
通讯作者：
Gamarnik AV
影响因子：
2.7
作者：
Liu, Y;Ford, BD;Fischbach, GD
通讯作者：
Fischbach, GD
DOI：
10.1073/pnas.0703348104
发表时间：
2007-05-22
影响因子：
11.1
作者：
Emara, Mohamed M.;Brinton, Margo A.
通讯作者：
Brinton, Margo A.
影响因子：
4.7
作者：
Bonenfant, Gaston;Meng, Ryan;Pager, Cara T.
通讯作者：
Pager, Cara T.
影响因子：
4.2
作者：
Bhagat, Reshma;Kaur, Guneet;Seth, Pankaj
通讯作者：
Seth, Pankaj