FREQUENT OCCURRENCE OF POLYOVULAR FOLLICLES IN OVARIES OF MICE EXPOSED NEONATALLY TO DIETHYLSTILBESTROL
FREQUENT OCCURRENCE OF POLYOVULAR FOLLICLES IN OVARIES OF MICE EXPOSED NEONATALLY TO DIETHYLSTILBESTROL
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DOI：
10.1002/tera.1420340105
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发表时间：
1986-08-01
期刊：
影响因子：
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通讯作者：
MILLS, KT
中科院分区：
文献类型：
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作者：
IGUCHI, T;TAKASUGI, N;MILLS, KT
BALB/cCrgl雌性小鼠从出生当天开始每天注射己烯雌酚(DES)0.1微克、己烯雌酚(DES)2微克、孕酮(P)100微克、己酸羟孕酮(HPC)137微克、睾酮(T)20微克、5α-双氢睾酮(DHT)20微克，或单独用油，观察10-34日龄BALB/cCrg1雌性小鼠卵巢中多卵泡卵泡(PF)的出现。以及接受5次新生儿注射的C57BL/Tw女性，1微克DES，20微克17β-雌二醇(E_2)，50微克5α-双羟色胺，50微克5-双羟色胺，或单独使用赋形剂。从3日龄至25日龄开始，每天注射5次1微克DES的30日龄C57BL小鼠的卵巢也被检查。注射DES、P、HPC和T的BALB/C小鼠的PF发生率(每卵巢PF的百分比)和PF频率(PF小鼠的百分比)显著高于对照组。34天时，DES处理组小鼠的PF发生率(2-13个卵母细胞/卵泡)是对照组的120-340倍。经T、P和HPC处理的BALB/c小鼠的PF发生率(2到4个卵母细胞/卵泡)是对照组的3到6倍。在30天龄的C57BL小鼠中，接受T、E2和DES治疗的小鼠，PF的发生率也增加了两到50倍。5.α-二羟色胺和5-β-二羟色胺不能增加PF的发生率。仅当新生儿DES治疗在0-3天开始时，PF的发生率才显著增加，但在10-25天开始治疗时，PF的发生率降低。在10-30日龄经DES处理的C57BL小鼠中，PF的发生率显著高于年龄匹配的对照组。
The occurrence of polyovular follicles (PF) was examined at 10-34 days of age in the ovaries of BALB/cCrgl female mice given five daily injections of 0.1 .mu.g diethylstilbestrol (DES), 2 .mu.g DES, 100 .mu.g progesterone (P), 137 .mu.g 17.alpha.-hydroxyprogesterone caproate (HPC), 20 .mu.g testosterone (T), 20 .mu.g 5.alpha.-dihydrotestosterone (5.sbd.DHT), or oil vehicle alone starting on the day of birth, and of C57BL/Tw females given five neonatal injections of 1 .mu.g DES, 20 .mu.g 17.beta.-estradiol (E2), 50 .mu.g 5.alpha.-DHT, 50 .mu.g 5.beta.-DHT, or the vehicle alone. Ovaries of 30-day-old C57BL mice given five daily injections of 1.mu.g DES starting at 3-25 days of age were also examined. PF incidence (% of PF per ovary) and PF frequency (% of mice with PF) were significantly greater in BALB/C mice receiving injections of DES, P, HPC, and T than in the controls. In DES-treated mice at 34 days, PF incidence (2-13 oocytes/follicle) was 120-340 times higher than in the controls. BALB/c mice treated with T, P, and HPC showed PF incidence (two to four oocytes/follicle) three- to six-fold higher than in the controls. In 30-day-old C57BL mice treated with T, E2, and DES, PF incidence also increased by two- to 50-fold. 5.alpha.-DHT and 5.beta.-DHT failed to increase PF incidence. PF incidence was significantly increased only when neonatal DES treatment was begun on days 0 to 3, but was reduced when started at days 10-25. In DES-treated C57BL mice aged 10-30 days, PF incidence was significantly higher than in the age-matched controls.