Unravelling vaginal microbial genetic diversity and abundance between Holstein and Fleckvieh cattle
Unravelling vaginal microbial genetic diversity and abundance between Holstein and Fleckvieh cattle
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揭示荷斯坦牛和弗莱克维牛之间阴道微生物遗传多样性和丰度
DOI：
10.1039/c7ra10553c
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Lu, Wenfa
中科院分区：
文献类型：
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作者：
Nesengani, Lucky T.;Wang, Jun;Lu, Wenfa
对牛阴道微生物遗传多样性的研究有助于更好地了解牛的生理，这对改善牛的生殖健康具有重要的经济意义。然而，人们对影响阴道微生物群落的因素知之甚少。本研究的目的是利用元基因组学方法评估和比较荷斯坦牛和弗莱克维耶牛的微生物遗传多样性和丰度。荷斯坦和弗莱克维的主要细菌门是坚果类(51.70%~58.80%)、变形杆菌(16.70%~11.70%)和类杆菌(13.20%~19.50%)。梭杆菌的含量差异显著(p<；0.05)，荷斯坦牛的梭菌数量较多(9.73-0.81%)。在Achaea门，Eurya chaeota显著不同(p<；0.05)，在Fleckvieh比在荷斯坦牛(0.86-0.22%)更丰富。在属水平上，Turicibacter、瘤胃球菌科_UCG-010、感觉严格梭状芽孢杆菌_1、DOREA、甲烷短杆菌、醋酸菌、瘤胃球菌科_NK4A214_群和瘤胃杆菌_5在两个黄牛品种间差异显著(p<；0.05)，且Fleckvieh均比荷斯坦牛丰富。目前的结果表明，品种可以影响牛阴道微生物的丰度和多样性。我们的研究描述并提供了关键的科学知识，以了解蕴藏牛处女菌群的微生物群落。
Characterization of bovine vaginal microbial genetic diversity can lead to better understanding of cattle physiology, which is of economic interest in improving cattle reproduction health. However, there is limited knowledge on the factors affecting the microbial community harbored in the vaginal tract. The aim of the current study is to evaluate and compare the microbial genetic diversity and abundance between the Holstein and Fleckvieh cattle using metagenomic approach. The main bacterial phyla found were firmicutes (51.70–58.80%), proteobacteria (16.70–11.70%) and bacteroidetes (13.20–19.50%) for Holstein and Fleckvieh. Notable significant difference (p < 0.05) was observed for Fusobacteria, which was more abundant in Holstein (9.73–0.81%). At the achaea phyla, Euryarchaeota was significantly different (p < 0.05) and was more abundant in Fleckvieh than in Holstein cattle (0.86–0.22%). At the genus level, Turicibacter, Ruminococcaceae_UCG-010, Clostridium_sensu_stricto_1, Dorea, Methanobrevibacter, Acetitomaculum, Ruminococcaceae_NK4A214_group and Ruminiclostridium_5 were found to be significantly (p < 0.05) different between the two cattle breeds and all of them were more abundant in Fleckvieh than in Holstein cattle. The current results indicate that the breed can influence bovine vaginal microbial abundance and diversity. Our study characterized and provided pivotal scientific knowledge to understand the microbial community that harbors the bovine virginal flora.