RNA REGULATORY ELEMENTS MEDIATE CONTROL OF DROSOPHILA BODY PATTERN BY THE POSTERIOR MORPHOGEN NANOS
RNA REGULATORY ELEMENTS MEDIATE CONTROL OF DROSOPHILA BODY PATTERN BY THE POSTERIOR MORPHOGEN NANOS
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DOI：
10.1016/0092-8674(91)90368-9
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发表时间：
1991-11-29
期刊：
影响因子：
64.5
通讯作者：
STRUHL, G
中科院分区：
文献类型：
--
作者：
WHARTON, RP;STRUHL, G
在果蝇胚胎中，后决定簇nanos（nos）的分级活性通过阻断hunchback（hb）基因的母体转录物的蛋白质表达来产生腹部分割。当在前极不适当地活跃时，nos也可以阻断前决定簇bicoid（bcd）的表达。我们表明，这两种调控相互作用是由类似的序列在3'非翻译区的每个转录本。这些nos反应元件（NRE）是赋予nos依赖性调节的必要和充分条件，调节的程度由元件的数量和质量以及体内nos的水平决定。基于这些和其他结果，我们认为，一氧化氮合酶作为一种形态发生素，控制血红蛋白的表达（因此腹部模式）作为其浓度依赖性的相互作用与NRE的功能。
In Drosophila embryos, graded activity of the posterior determinant nanos (nos) generates abdominal segmentation by blocking protein expression from maternal transcripts of the hunchback (hb) gene. When active inappropriately at the anterior pole, nos can also block expression of the anterior determinant bicoid (bcd). We show that both regulatory interactions are mediated by similar sequences in the 3' untranslated region of each transcript. These nos response elements (NREs) are both necessary and sufficient to confer nos-dependent regulation, the degree of regulation determined by the number and quality of the elements and the level of nos in vivo. Based on these and other results, we argue that nos acts as a morphogen, controlling hb expression (and hence abdominal pattern) as a function of its concentration-dependent interaction with the NREs.