Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients.
Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients.
复制标题
DOI：
10.3390/v12111314
复制
发表时间：
2020-11-17
期刊：
影响因子：
--
通讯作者：
Goneau LW
中科院分区：
文献类型：
--
作者：
Kandel C;Zheng J;McCready J;Serbanescu MA;Racher H;Desaulnier M;Powis JE;Vojdani K;Finlay L;Sheldrake E;Vermeiren C;Katz K;McGeer A;Kozak R;Goneau LW
Widely available and easily accessible testing for COVID-19 is a cornerstone of pandemic containment strategies. Nasopharyngeal swabs (NPS) are the currently accepted standard for sample collection but are limited by their need for collection devices and sampling by trained healthcare professionals. The aim of this study was to compare the performance of saliva to NPS in an outpatient setting. This was a prospective study conducted at three centers, which compared the performance of saliva and NPS samples collected at the time of assessment center visit. Samples were tested by real-time reverse transcription polymerase chain reaction and sensitivity and overall agreement determined between saliva and NPS. Clinical data was abstracted by chart review for select study participants. Of the 432 paired samples, 46 were positive for SARS-CoV-2, with seven discordant observed between the two sample types (four individuals testing positive only by NPS and three by saliva only). The observed agreement was 98.4% (kappa coefficient 0.91) and a composite reference standard demonstrated sensitivity of 0.91 and 0.93 for saliva and NPS samples, respectively. On average, the Ct values obtained from saliva as compared to NPS were higher by 2.76. This study demonstrates that saliva performs comparably to NPS for the detection of SARS-CoV-2. Saliva was simple to collect, did not require transport media, and could be tested with equipment readily available at most laboratories. The use of saliva as an acceptable alternative to NPS could support the use of widespread surveillance testing for SARS-CoV-2.
登录
查看更多内容
影响因子：
3.9
作者：
Czumbel, Laszlo Mark;Kiss, Szabolcs;Varga, Gabor
通讯作者：
Varga, Gabor
影响因子：
11.8
作者：
To, Kelvin Kai-Wang;Tsang, Owen Tak-Yin;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung
中国武汉市 138 例 2019 新型冠状病毒感染肺炎住院患者的临床特征。
影响因子：
120.7
作者：
Wang, Dawei;Hu, Bo;Peng, Zhiyong
通讯作者：
Peng, Zhiyong
影响因子：
82.9
作者：
Long, Quan-Xin;Tang, Xiao-Jun;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
影响因子：
11.8
作者：
Rao, Mohan;Rashid, Fairuz A.;Ahmad, Norazah
通讯作者：
Ahmad, Norazah