CLONING OF THE CDNA AND GENE FOR A HUMAN D2-DOPAMINE RECEPTOR
CLONING OF THE CDNA AND GENE FOR A HUMAN D2-DOPAMINE RECEPTOR
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DOI：
10.1073/pnas.86.24.9762
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发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
CIVELLI, O
中科院分区：
文献类型：
--
作者：
GRANDY, DK;MARCHIONNI, MA;CIVELLI, O
从垂体cDNA文库中分离到一个编码人类D2多巴胺受体的克隆，并对其进行测序。推断出的蛋白质序列与克隆的大鼠受体96%相同，但有一个主要区别：人类受体在其假定的第三细胞质环中含有额外的29个氨基酸。Southern blotting显示仅存在一个人类D2受体基因。分离并鉴定了两个重叠的噬菌体。对这些克隆的DNA序列分析表明，编码序列被6个内含子中断，并且存在于人垂体受体中的额外氨基酸由单个87个碱基对的外显子编码。讨论了该序列在选择性剪接中的作用及其生物学意义。
A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.