A gradient HPLC method for the quality control of chlorogenic acid, linarin and luteolin in Flos Chrysanthemi Indici suppository
A gradient HPLC method for the quality control of chlorogenic acid, linarin and luteolin in Flos Chrysanthemi Indici suppository
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DOI：
10.1016/j.jpba.2006.07.037
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发表时间：
2007-01-17
影响因子：
3.4
通讯作者：
Cheng, Wenming
中科院分区：
文献类型：
--
作者：
Zhang, Qunlin;Li, Jun;Cheng, Wenming
野菊花栓是一种著名的传统中药，用于治疗前列腺炎和慢性盆腔炎。建立了一种简便、快速、准确的高效液相色谱法同时测定野菊花栓中绿原酸、蒙花苷和木犀草素的含量。采用Hypersil ODS色谱柱，以乙腈和1.0%乙酸水溶液为移动的流动相，梯度洗脱，23 min内分离效果良好。日内和日间精密度均小于3.0%，绿原酸、蒙花苷和木犀草素的检出限（S/N = 3）分别为0.32、0.08和0.05 μ g/ml。该方法可用于测定山银花栓剂中3种有效酚类化合物的含量，回收率在94.7%~ 101.8%之间。结果表明，所建立的HPLC法可用于山银花栓剂及其相关中药制剂的质量控制。(c)2006 Elsevier B. V.保留所有权利。
The Flos Chrysanthemi Indici suppository, a famous traditional Chinese medicine, is marketed for the treatment of prostatitis and chronic pelvic inflammation. A simple, relatively rapid and accurate high-performance liquid chromatographic (HPLC) method was applied to the simultaneous determination of chlorogenic acid, linarin and luteolin in Flos Chrysanthemi Indici suppository. The good separation was achieved on a Hypersil ODS column by a gradient elution using the mixture of acetonitrile and aqueous 1.0% acetic acid as mobile phase within 23 min. The method was validated for three active phenolic compounds with the relative standard deviations (R.S.D.) of intra- and inter-day precision below 3.0%, and the detection limits (S/N = 3) of chlorogenic acid, linarin and luteolin were 0.32, 0.08 and 0.05 mu g ml(-1), respectively. This assay was successfully applied to the determination of three active phenolic compounds in Flos Chrysanthemi Indici suppository samples with the quantitative recoveries in the range of 94.7-101.8%. The results indicate that the developed HPLC method can be readily utilized as a quality control method for Flos Chrysanthemi Indici suppository and its related traditional Chinese medicinal preparations. (c) 2006 Elsevier B.V. All rights reserved.