Photo-click living strategy for controlled, reversible exchange of biochemical ligands.
Photo-click living strategy for controlled, reversible exchange of biochemical ligands.
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DOI：
10.1002/adma.201304847
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发表时间：
2014-04-23
期刊：
影响因子：
--
通讯作者：
Anseth KS
中科院分区：
文献类型：
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作者：
Gandavarapu NR;Azagarsamy MA;Anseth KS
合成水凝胶越来越多地被设计用于提供和重现体内细胞周围细胞外基质（ECM）的复杂动态环境。令人感兴趣的是化学策略的设计，不仅可以制造可用作二维（2d）和三维（3d）细胞培养系统的细胞培养平台的水凝胶，而且还可以结合模拟 ECM 几个关键方面的生物活性特征。 [1]–[4] 一类基于聚（乙二醇）（PEG）的合成水凝胶已成为一种多样化的水凝胶。 合成 ECM 模拟物，[5] 因为 PEG 本质上是亲水性的，可在市场上以各种分子量和结构购买，并且具有与许多软组织相似的材料特性。此外，为了补充其生物物理特性，PEG 水凝胶的相对生物惰性提供了整合各种生化线索并研究其对宿主细胞的影响的机会，同时最大限度地减少血清蛋白的混淆非特异性相互作用。因此，人们对用生物活性部分（包括小分子、肽和蛋白质）功能化 PEG 水凝胶的策略越来越感兴趣，旨在指导和操纵细胞功能，包括粘附、增殖、分化、运动等。但除了生物功能材料的合成之外，最近的努力还集中在概括 ECM 的动态性质，这通常需要 对生化信号呈现的多维控制。例如，研究表明，肽基序的掺入可促进细胞粘附[6]-[9]（例如，纤连蛋白衍生的
Synthetic hydrogels have been increasingly engineered to provide and recapitulate the complex and dynamic environment of extra-cellular matrix (ECM) surrounding cells in vivo. Of significant interest is the design of chemical strategies not only to fabricate hydrogels that can function as cell culture platforms for both two-dimensional (2d) and threedimensional (3d) cell culture systems, but also to incorporate biologically active features that mimic several critical aspects of the ECM.[1]–[4] One class of synthetic hydrogels, based on poly (ethylene glycol)(PEG), has emerged as a diverse synthetic ECM mimic,[5] as PEGs are hydrophilic in nature, commercially available in a range of molecular weights and structures, and possess materials properties similar to many soft tissues. Further, to complement their biophysical properties, the relative bio-inertness of PEG hydrogels renders opportunities to incorporate various biochemical cues and study their effects on host cells, while minimizing confounding non-specific interactions from serum proteins. Hence, there is a growing interest in strategies to functionalize PEG hydrogels with bioactive moieties, including small molecules, peptides, and proteins, aimed at directing and manipulating cellular functions ranging from adhesion, proliferation, differentiation, motility, etc.But beyond the synthesis of biofunctional materials, recent efforts are focused on recapitulating the dynamic nature of ECM, which often requires multidimensional control over the presentation of the biochemical signals. For example, studies have demonstrated incorporation of peptides motifs that facilitate cell adhesion [6]–[9](eg, fibronectin derived