SCRAPIE AGENT CONTAINS A HYDROPHOBIC PROTEIN
SCRAPIE AGENT CONTAINS A HYDROPHOBIC PROTEIN
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DOI：
10.1073/pnas.78.11.6675
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
MASIARZ, FR
中科院分区：
文献类型：
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作者：
PRUSINER, SB;MCKINLEY, MP;MASIARZ, FR
痒病剂会引起绵羊和山羊的神经系统退行性疾病。大量证据表明痒病剂含有感染性所必需的蛋白质，但由于缺乏足够纯化的制剂，直接证明蛋白质部分受到阻碍。使用蛋白质含量丰富 100 至 1000 倍的痒病剂制剂，用蛋白酶 K 消化可破坏 > 99.9% 的感染性。焦碳酸二乙酯可以高效地对蛋白质中的氨基酸残基进行化学修饰，也可以使这些纯化制剂中的痒病剂失活。对于纯化程度较低的制剂，未观察到蛋白酶 K 和焦碳酸二乙酯导致的感染性降低。与苯基-Sepharose结合的试剂不能用8.5M乙二醇洗脱；乙二醇和清洁剂的组合确实释放了该试剂。因此，提供了瘙痒剂内的蛋白质和疏水结构域的证据。感染性所需的蛋白质是否与负责瘙痒病剂疏水性的蛋白质相同，仍有待确定。
The scrapie agent causes a degenerative nervous system disorder of sheep and goats. Considerable evidence indicates that the scrapie agent contains a protein that is necessary for infectivity, but direct demonstration of a protein moiety has been hampered by lack of sufficiently purified preparations. Employing preparations of the scrapie agent enriched 100- to 1000-fold with respect to protein, digestion by proteinase K destroyed > 99.9% of the infectivity. Diethylpyrocarbonate, which chemically modifies amino acid residues in proteins with high efficiency, also inactivated the scrapie agent in these purified preparations. Reductions of infectivity by proteinase K and diethylpyrocarbonate were not observed with less purified preparations. The agent bound to phenyl-Sepharose could not be eluted with 8.5 M ethylene glycol; a combination of ethylene glycol and detergents did release the agent. Thus evidence for a protein and for hydrophobic domains within the scrapie agent is provided. Whether the protein required for infectivity is the same protein responsible for the hydrophobic properties of the scrapie agent remains to be established.