A pre-clinical assessment model of rat autogeneic bone marrow stromal cell transplantation into the central nervous system
A pre-clinical assessment model of rat autogeneic bone marrow stromal cell transplantation into the central nervous system
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DOI：
10.1016/j.brainresprot.2004.09.004
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发表时间：
2004-11-01
期刊：
影响因子：
--
通讯作者：
Iwasaki, Y
中科院分区：
文献类型：
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作者：
Lee, JB;Kuroda, S;Iwasaki, Y
为了验证“自体”骨髓基质细胞（BMSC）移植治疗神经系统疾病的生物学方面，我们的研究旨在评估自体BMSC在中枢神经系统（CNS）中的存活、分布和分化。我们收获大鼠骨髓间充质干细胞从股骨骨，细胞核荧光标记的24小时共培养与双-苯甲亚胺。将这些BMSC立体定向注射到每只动物的纹状体（n=6）或胸髓（n=8）中。4周后，我们用免疫组化技术评估了“自体”BMSC在脑和脊髓中的分布和分化。我们在同侧纹状体、海马、新皮质和双侧胼胝体中发现了一些注射的细胞，大约20%和15%的移植细胞分别表达神经元和星形胶质细胞标记物。其他注射的细胞分布在背索和邻近的灰质中，这些细胞中分别约有10%和15%表达神经元和星形胶质细胞标记物。虽然BMSC转分化的确切机制仍不清楚，但目前的研究结果表明，“自体”BMSC可以高度分化为自己的CNS神经细胞，这表明它们周围有有利的条件。(C)2004 Elsevier B. V.保留所有权利。
In order to verify the biological aspects of 'autogeneic' bone marrow stromal cells (BMSC) transplantation for neurological disorders, we aimed our study towards the assessment of the survival, distribution, and differentiation of autologous BMSC in the central nervous system (CNS). We harvested rat BMSC from femur bones, and the nuclei were then fluorescently labeled by a 24-h co-culture with bis-benzimide. These BMSC were stereotactically injected into the striatum (n=6) or thoracic cord (n=8) of each animal. We evaluated the distribution and differentiation of 'autogeneic' BMSC in the brain and spinal cord after 4 weeks, using the immunohistochemistry technique. We found some injected cells in the ipsilateral striatum, hippocampus, neocortex, and bilateral corpus callosum, and approximately 20% and 15% of the engrafted cells expressed neuronal and astrocytic markers, respectively. Other injected cells were distributed in the dorsal funiculus and adjacent gray matter, and about 10% and 15% of these cells expressed neuronal and astrocytic markers, respectively. Although the precise mechanism of BMSC transdifferentiation still remains unclear, the present results show that 'autogeneic' BMSC could highly differentiate into their own CNS neural cells, suggesting that they are surrounded by favorable conditions. (C) 2004 Elsevier B.V. All rights reserved.